Wheat gluten hydrolysates (WGH) were used to examine their adsorption-desorption kinetics and thermodynamics characteristics on six macroporous resins, and their effects on the stress tolerance in brewer's yeast. Results showed that the pseudo second-order kinetics, Langmuir and Freundlich model could illuminate the adsorption mechanism, and the adsorption process was physical, spontaneous and exothermic. The 40% ethanol fraction separated by XAD-16 resin improved significantly the ethanol tolerance and the viability of brewer's yeast, while the 0% ethanol fraction separated by XAD-16 resin increased obviously the osmotic stress tolerance and the viability of brewer's yeast. Results from scanning electron microscopy showed that both these WGH fractions could increase budding rate and maintain normal morphology of yeast cells under various environmental stress. Thus, WGH separated by macroporous resin could be used in high gravity brewing to enhance the ethanol and osmotic stress tolerance in brewer's yeast.
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Evidence ID | Analyze ID | Gene/Complex | Systematic Name/Complex Accession | Qualifier | Gene Ontology Term ID | Gene Ontology Term | Aspect | Annotation Extension | Evidence | Method | Source | Assigned On | Reference |
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Evidence ID | Analyze ID | Gene | Gene Systematic Name | Phenotype | Experiment Type | Experiment Type Category | Mutant Information | Strain Background | Chemical | Details | Reference |
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Evidence ID | Analyze ID | Gene | Gene Systematic Name | Disease Ontology Term | Disease Ontology Term ID | Qualifier | Evidence | Method | Source | Assigned On | Reference |
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Evidence ID | Analyze ID | Regulator | Regulator Systematic Name | Target | Target Systematic Name | Direction | Regulation of | Happens During | Regulator Type | Direction | Regulation Of | Happens During | Method | Evidence | Strain Background | Reference |
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Site | Modification | Modifier | Source | Reference |
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Evidence ID | Analyze ID | Interactor | Interactor Systematic Name | Interactor | Interactor Systematic Name | Allele | Assay | Annotation | Action | Phenotype | SGA score | P-value | Source | Reference | Note |
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Evidence ID | Analyze ID | Interactor | Interactor Systematic Name | Interactor | Interactor Systematic Name | Assay | Annotation | Action | Modification | Source | Reference | Note |
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Complement ID | Locus ID | Gene | Species | Gene ID | Strain background | Direction | Details | Source | Reference |
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Evidence ID | Analyze ID | Dataset | Description | Keywords | Number of Conditions | Reference |
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