During meiosis, formation of crossovers-the physical links that ensure the segregation of homologous chromosomes-requires a group of evolutionarily conserved ZMM proteins. In budding yeast, three ZMM proteins, ZIP2, SPO16, and Zip4, form a trimeric complex to bind recombination intermediates and promote crossover formation. Here, we show that MZIP2 is the mammalian ortholog of ZIP2. Complete ablation of MZIP2 in mice caused sterility in both males and females, as well as defects in repairing meiotic DNA double-strand breaks. MZIP2 forms discrete foci on chromosomes axes, and is required for the localization of TEX11 (mammalian Zip4 ortholog) and another ZMM protein, MSH4, to form crossover-prone recombination intermediates. As a consequence, formation of crossovers is abolished and formation of synaptonemal complex is incomplete in MZIP2-null meiocytes, resulting in meiosis arrest at a zygotene-like stage. Our results suggest that the processing of early recombination intermediates toward mature crossovers is dependent on MZIP2.
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| Evidence ID | Analyze ID | Gene/Complex | Systematic Name/Complex Accession | Qualifier | Gene Ontology Term ID | Gene Ontology Term | Aspect | Annotation Extension | Evidence | Method | Source | Assigned On | Reference |
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| Evidence ID | Analyze ID | Gene | Gene Systematic Name | Phenotype | Experiment Type | Experiment Type Category | Mutant Information | Strain Background | Chemical | Details | Reference |
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| Evidence ID | Analyze ID | Gene | Gene Systematic Name | Disease Ontology Term | Disease Ontology Term ID | Qualifier | Evidence | Method | Source | Assigned On | Reference |
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| Evidence ID | Analyze ID | Regulator | Regulator Systematic Name | Target | Target Systematic Name | Direction | Regulation of | Happens During | Regulator Type | Direction | Regulation Of | Happens During | Method | Evidence | Strain Background | Reference |
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| Site | Modification | Modifier | Source | Reference |
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| Evidence ID | Analyze ID | Interactor | Interactor Systematic Name | Interactor | Interactor Systematic Name | Allele | Assay | Annotation | Action | Phenotype | SGA score | P-value | Source | Reference | Note |
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| Evidence ID | Analyze ID | Interactor | Interactor Systematic Name | Interactor | Interactor Systematic Name | Assay | Annotation | Action | Modification | Source | Reference | Note |
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| Complement ID | Locus ID | Gene | Species | Gene ID | Strain background | Direction | Details | Source | Reference |
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| Evidence ID | Analyze ID | Dataset | Description | Keywords | Number of Conditions | Reference |
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| Evidence ID | Analyze ID | File | Description |
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