Malachite green (MG), as a triarylmethane compound, poses a health hazard and causes considerable environmental concern. In this work, batch biosorption experiments were conducted under different operational conditions such as pH, contact time and adsorption dose to assess the optimal parameters of MG dye removal by yeast biomass from aqueous solutions. Then, the conventional biochemical assay was used to evaluate MG removal efficiency (75.18 and 95.85%) by Saccharomyces cerevisiae and Candida utilis. In addition, Fourier-transform infrared spectroscopy in combination with Raman microspectroscopy was employed to scrutinize the differences of dye removal between two types of yeast strains. This study demonstrates that Raman microspectroscopy may serve as a useful and powerful tool to quantitatively measure the content of MG dye on yeast cell surfaces in situ, and even offer an alternative new technique to seek potentially proper adsorbents for the removal of toxic dyes from industrial effluents.
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| Evidence ID | Analyze ID | Gene/Complex | Systematic Name/Complex Accession | Qualifier | Gene Ontology Term ID | Gene Ontology Term | Aspect | Annotation Extension | Evidence | Method | Source | Assigned On | Reference |
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| Evidence ID | Analyze ID | Gene | Gene Systematic Name | Phenotype | Experiment Type | Experiment Type Category | Mutant Information | Strain Background | Chemical | Details | Reference |
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| Evidence ID | Analyze ID | Gene | Gene Systematic Name | Disease Ontology Term | Disease Ontology Term ID | Qualifier | Evidence | Method | Source | Assigned On | Reference |
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| Evidence ID | Analyze ID | Regulator | Regulator Systematic Name | Target | Target Systematic Name | Direction | Regulation of | Happens During | Regulator Type | Direction | Regulation Of | Happens During | Method | Evidence | Strain Background | Reference |
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| Site | Modification | Modifier | Source | Reference |
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| Evidence ID | Analyze ID | Interactor | Interactor Systematic Name | Interactor | Interactor Systematic Name | Allele | Assay | Annotation | Action | Phenotype | SGA score | P-value | Source | Reference | Note |
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| Evidence ID | Analyze ID | Interactor | Interactor Systematic Name | Interactor | Interactor Systematic Name | Assay | Annotation | Action | Modification | Source | Reference | Note |
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| Complement ID | Locus ID | Gene | Species | Gene ID | Strain background | Direction | Details | Source | Reference |
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| Evidence ID | Analyze ID | Dataset | Description | Keywords | Number of Conditions | Reference |
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