Using Saccharomyces cerevisiae as an experimental model, the potential toxicological effects of Fe₃O₄ nanoparticles (Fe₃O₄-NPs) were investigated following exposure to 0-600 mg/L for 24 h. Results revealed that cell proliferation was significantly inhibited by Fe₃O₄-NPs with an IC₅₀ value of 326.66 mg/L. Mortality showed a concentration-dependent increase, and the highest concentration in this study (600 mg/L) resulted in 22.30% mortality. In addition, Effects on proliferation and mortality were accounted for Fe₃O₄-NPs rather than iron ion released from Fe₃O₄-NPs. Scanning and transmission electron microscope observation showed that Fe₃O₄-NPs extensively attached on the cell surfaces, causing cells to deform and shrink. Moreover, Fe₃O₄-NPs could be internalized in S. cerevisiae cells via endocytosis and then be distributed in cytoplasm and vesicles. The data of uptake kinetics demonstrated that the maximal accumulation (4.898 mg/g) was reached at 15 h. Besides, percentage of late apoptosis/necrosis was observably increased (p < 0.01) at 600 mg/L (15.80%), and the expression levels of apoptosis-related genes (SOD, Yca1 and Nuc1) were dramatically increased following exposure to Fe₃O₄-NPs for 24 h. As expected, mitochondrial transmembrane potential was significantly decreased (p < 0.01) at 50-600 mg/L, and biomarkers of oxidative stress (ROS, CAT and SOD) were also markedly changed following exposure. Altogether, the combined results so far indicated Fe₃O₄-NPs could induce S. cerevisiae cell apoptosis that mediated by mitochondrial impairment and oxidative stress.

• PMID: 31654831
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Journal Article

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Luo F, Zhu S, Hu Y, Yang KC, He MS, Zhu B, Wang GX, Ling F

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