In this study, the effect of soluble polysaccharides (SPs) derived from agricultural waste, rice straw, on fermentation-associated stresses (temperature and concentrations of glucose and ethanol) was investigated to achieve high-performance ethanol production. The increase in temperature and concentrations of glucose and ethanol significantly inhibited Saccharomyces cerevisiae growth and lowered ethanol fermentation efficiency. Flow cytometric assays indicated that SPs could alleviate membrane permeability damage caused by fermentation-associated stresses. Atomic force microscopy and transmission electron microscopy revealed that fermentation-associated stresses induced cell surface shrinkage, causing a decrease in the cell size, whereas SPs stimulated the formation of extracellular matrices (EMs), which made the cell surface smooth and the cell morphology regular. Cells with EMs induced by SPs could efficiently produce ethanol under severe stresses. As a result, the titer of ethanol in the fermentation with SPs was 1.40-fold (from 26.40 to 36.98 g/L) higher than that in the fermentation without SPs, suggesting the stress-alleviating effect of SPs on ethanol production.
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| Evidence ID | Analyze ID | Gene/Complex | Systematic Name/Complex Accession | Qualifier | Gene Ontology Term ID | Gene Ontology Term | Aspect | Annotation Extension | Evidence | Method | Source | Assigned On | Reference |
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| Evidence ID | Analyze ID | Gene | Gene Systematic Name | Phenotype | Experiment Type | Experiment Type Category | Mutant Information | Strain Background | Chemical | Details | Reference |
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| Evidence ID | Analyze ID | Gene | Gene Systematic Name | Disease Ontology Term | Disease Ontology Term ID | Qualifier | Evidence | Method | Source | Assigned On | Reference |
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| Evidence ID | Analyze ID | Regulator | Regulator Systematic Name | Target | Target Systematic Name | Direction | Regulation of | Happens During | Regulator Type | Direction | Regulation Of | Happens During | Method | Evidence | Strain Background | Reference |
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| Site | Modification | Modifier | Source | Reference |
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| Evidence ID | Analyze ID | Interactor | Interactor Systematic Name | Interactor | Interactor Systematic Name | Allele | Assay | Annotation | Action | Phenotype | SGA score | P-value | Source | Reference | Note |
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| Evidence ID | Analyze ID | Interactor | Interactor Systematic Name | Interactor | Interactor Systematic Name | Assay | Annotation | Action | Modification | Source | Reference | Note |
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| Complement ID | Locus ID | Gene | Species | Gene ID | Strain background | Direction | Details | Source | Reference |
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| Evidence ID | Analyze ID | Dataset | Description | Keywords | Number of Conditions | Reference |
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