The insolubility of Saccharomyces cerevisiae β-D-glucan in most common solvents seriously restricts its applications and recovery. In this work, ionic liquids (ILs) were used for dissolution and modification under high pressure microfluidization (HPM). ILs 1-ethyl-3-methylimidazolium acetate (EmimAc) showed good β-D-glucan dissolving capacities, which can facilitate its structural modifications to recycle under environmentally friendly conditions. However, the synergistic effect of ionic liquid and high pressure microfluidization on physiochemical properties and structure of S. cerevisiae β-D-glucan were unclearly. The objectives of this study were: 1) to study the physiochemical properties of β-D-glucan after treating by ILs and HPM; 2) to compare the essential structure and conformation of β-D-glucan after treating by ILs and HPM; 3) to provide theoretical basis for precise regulation and green manufacturing of soluble S. cerevisiae β-D-glucan.
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| Evidence ID | Analyze ID | Gene/Complex | Systematic Name/Complex Accession | Qualifier | Gene Ontology Term ID | Gene Ontology Term | Aspect | Annotation Extension | Evidence | Method | Source | Assigned On | Reference |
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| Evidence ID | Analyze ID | Gene | Gene Systematic Name | Phenotype | Experiment Type | Experiment Type Category | Mutant Information | Strain Background | Chemical | Details | Reference |
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| Evidence ID | Analyze ID | Gene | Gene Systematic Name | Disease Ontology Term | Disease Ontology Term ID | Qualifier | Evidence | Method | Source | Assigned On | Reference |
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| Evidence ID | Analyze ID | Regulator | Regulator Systematic Name | Target | Target Systematic Name | Direction | Regulation of | Happens During | Regulator Type | Direction | Regulation Of | Happens During | Method | Evidence | Strain Background | Reference |
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| Site | Modification | Modifier | Source | Reference |
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| Evidence ID | Analyze ID | Interactor | Interactor Systematic Name | Interactor | Interactor Systematic Name | Allele | Assay | Annotation | Action | Phenotype | SGA score | P-value | Source | Reference | Note |
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| Evidence ID | Analyze ID | Interactor | Interactor Systematic Name | Interactor | Interactor Systematic Name | Assay | Annotation | Action | Modification | Source | Reference | Note |
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| Complement ID | Locus ID | Gene | Species | Gene ID | Strain background | Direction | Details | Source | Reference |
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| Evidence ID | Analyze ID | Dataset | Description | Keywords | Number of Conditions | Reference |
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