To realize encapsulation of living microbial cells and easily evaluation of cell viability after immobilization, the yeast cells were encapsulated in water soluble PAAm nanofiber by a facile and effective electrospinning technology. Firstly, the conductivity, shear viscosity and surface tension of PAAm/yeast electrospinning solution as a function of mass ratios of yeast/PAAm were investigated to determine the optimum solution condition for electrospinning immobilization. After electrospinning, it is interesting to note that the original ellipsoidal structure of yeast cells turns to oblate spheroid structure. To distinguish immobilization structure from the bead appearing during general electrospinning process, immobilization structure and bead structure were compared and analyzed by FESEM and EDX. Free cell activity, the immediate cell activity after electrospinning and cell activity for seven days storage after immobilization were evaluated by dying methods of CTC and methylene blue, respectively. The results show that encapsulation efficiency maintained at about 40%, and immobilized yeast cells remain active even after seven days storage, which provides a promising application prospect for electrospinning immobilization.
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| Evidence ID | Analyze ID | Gene/Complex | Systematic Name/Complex Accession | Qualifier | Gene Ontology Term ID | Gene Ontology Term | Aspect | Annotation Extension | Evidence | Method | Source | Assigned On | Reference |
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| Evidence ID | Analyze ID | Gene | Gene Systematic Name | Phenotype | Experiment Type | Experiment Type Category | Mutant Information | Strain Background | Chemical | Details | Reference |
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| Evidence ID | Analyze ID | Gene | Gene Systematic Name | Disease Ontology Term | Disease Ontology Term ID | Qualifier | Evidence | Method | Source | Assigned On | Reference |
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| Evidence ID | Analyze ID | Regulator | Regulator Systematic Name | Target | Target Systematic Name | Direction | Regulation of | Happens During | Regulator Type | Direction | Regulation Of | Happens During | Method | Evidence | Strain Background | Reference |
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| Site | Modification | Modifier | Source | Reference |
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| Evidence ID | Analyze ID | Interactor | Interactor Systematic Name | Interactor | Interactor Systematic Name | Allele | Assay | Annotation | Action | Phenotype | SGA score | P-value | Source | Reference | Note |
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| Evidence ID | Analyze ID | Interactor | Interactor Systematic Name | Interactor | Interactor Systematic Name | Assay | Annotation | Action | Modification | Source | Reference | Note |
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| Complement ID | Locus ID | Gene | Species | Gene ID | Strain background | Direction | Details | Source | Reference |
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| Evidence ID | Analyze ID | Dataset | Description | Keywords | Number of Conditions | Reference |
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| Evidence ID | Analyze ID | File | Description |
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