In this study, a novel adsorbent, Air Environment-prepared Adsorbent at 250 ℃ (AEPA₂₅₀), was used to detoxify the main fermentation inhibitor (ferulic acid) present in the alkali-pretreated hydrolysate. AEPA₂₅₀ reduced the effective concentration of ferulic acid by its adsorption, thereby decreasing the possible interaction of ferulic acid with Saccharomyces cerevisiae. The results indicated that AEPA₂₅₀ functionalized with hydroxyl, carboxyl, and amino groups under acidic conditions with higher binding energies (-45.667, -27.046, and -11.008 kcal mol^-1, respectively) and electronic cloud overlap and shorter bond distances (1.015, 1.010, and 2.094 Å, respectively) than those under the other pH conditions. These differences revealed that the electrostatic interaction dominated ferulic acid adsorption on AEPA₂₅₀. Additionally, under acidic conditions and for carboxyl group functionalized AEPA₂₅₀, energy band gap values of Eg1 were higher than those of Eg2, indicating that ferulic acid provided the π-electrons for the π-π electron donor-acceptor interactions with AEPA₂₅₀. Furthermore, ferulic acid detoxification after AEPA₂₅₀ adsorption caused the regulation of YDR316W-B and YPR137C-B genes of S. cerevisiae. These results might contribute to the development of other more efficient adsorbents and pretreatment methods and allow yeast engineering for improving the scale-up and self-sufficient production of bioethanol in the future.

• PMID: 34088183
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Journal Article | Research Support, Non-U.S. Gov't

Authors

Hou J, Zhang S, Zhang X, Wang K, Zhang Q, Shi Y

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