Reference: Bi X, et al. (2021) Site-Selective and Biocompatible Growth of Polymers from Glycan Moieties of Glycoproteins and Living Cells. Biomacromolecules 22(10):4237-4243

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Abstract


Formation of protein-polymer conjugates (PPCs) is critical for many studies in chemical biology, biomedicine, and enzymatic catalysis. Polymers with coordinated physicochemical properties confer synergistic functions to PPCs that overcome the inherent limitation of proteins. However, application of PPCs has been synthetically restricted by the limited modification sites and polymer grafting method. Here, we present a versatile strategy for site-selective PPC synthesis. The initiator was specifically tethered to the preoxidized glycan moieties through oxime chemistry. Polymer brushes were grown in situ from the glycan by atom-transfer radical polymerization to generate well-controlled PPCs. Notably, the modification is site-specific, multivalent, and alterable depending on protein glycosylation. Additionally, we demonstrated that the cytocompatible method enabled the growth of polymer chains from the surface of living yeast cells. These results verified a facile technology for surface modification of biomacromolecules by desired polymers for various biomedical applications.

Reference Type
Journal Article | Research Support, Non-U.S. Gov't
Authors
Bi X, Xiong W, He J, Ma S, Zhang J, Fang Y, Wu Y
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