The CGA codon is a rare codon in Saccharomyces cerevisiae and is known to be inefficiently decoded by wobble pairing with Arg-tRNA(ICG). The tRNA^Arg (ICG) is post-transcriptionally edited from tRNA^Arg (ACG) by the anticodon first adenosine deamination enzyme Tad2/Tad3 complex. Experimental consecutive CGA codons cause ribosome stalling to result in the reduction of the encoding protein product. In this study, the additional supply of tRNA^Arg (ACG) genes that produce decoding Arg-tRNA(ICG) promoted the product level from the CGA12-luc reporter, revealing that the product reduction is essentially due to inefficient decoding and deficiency in the tRNA supply. The mature tRNA^Arg (ICG) and the precursor tRNA^Arg (ACG) ratios examined for cellular tRNA fraction revealed that the tRNA^Arg (ICG) ratio is maintained at less than 30% and is responsive to the Tad2/Tad3 expression level.

• PMID: 36806932
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Reference Type

Journal Article | Research Support, Non-U.S. Gov't

Authors

Wada M, Ito K

Primary Lit For

YNCE0028C | YNCE0010W | YNCJ0008W | HSX1 | YNCJ0011W | YNCJ0014C | YNCD0013C | YNCD0011W | YNCG0032W | TAD2 | YNCL0007W | ... Show allTRR4 | YNCB0015W | YNCG0017W | YNCG0038C | YNCK0017W | YNCK0006C | YNCQ0017W | YNCQ0013W | TAD3 | YNCO0012C | YNCM0033C | YNCM0004C Show fewer

Additional Lit For

Review For