Precise and efficient manipulation of gene expression or rewriting genome sequence is the research hotspots of genome editing, and it is also the core enabling technology contributing to the rapid development of industrial biotechnology. Genome editing technology has experienced three stages of development, from zinc finger nuclease (ZFNs), to transcription activator like effector nuclease (TALEN) and Cas nuclease. Currently, vigorous development of CRISPR/Cas has enabled researchers establish a series of first-generation and second-generation Cas-based genome editing technologies. This contributed to the establishment and optimization for prokaryotic chassis such as Escherichia coli or eukaryotic chassis such as Saccharomyces cerevisiae. This paper summarizes the current development and application of industrial biotechnology using conventional chassis cells, and prospects future development trend with the aim to facilitate researchers to optimize industrial biotechnology and its potential applications.
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| Evidence ID | Analyze ID | Gene/Complex | Systematic Name/Complex Accession | Qualifier | Gene Ontology Term ID | Gene Ontology Term | Aspect | Annotation Extension | Evidence | Method | Source | Assigned On | Reference |
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| Evidence ID | Analyze ID | Gene | Gene Systematic Name | Phenotype | Experiment Type | Experiment Type Category | Mutant Information | Strain Background | Chemical | Details | Reference |
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| Evidence ID | Analyze ID | Gene | Gene Systematic Name | Disease Ontology Term | Disease Ontology Term ID | Qualifier | Evidence | Method | Source | Assigned On | Reference |
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| Evidence ID | Analyze ID | Regulator | Regulator Systematic Name | Target | Target Systematic Name | Direction | Regulation of | Happens During | Regulator Type | Direction | Regulation Of | Happens During | Method | Evidence | Strain Background | Reference |
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| Site | Modification | Modifier | Source | Reference |
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| Evidence ID | Analyze ID | Interactor | Interactor Systematic Name | Interactor | Interactor Systematic Name | Allele | Assay | Annotation | Action | Phenotype | SGA score | P-value | Source | Reference | Note |
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| Evidence ID | Analyze ID | Interactor | Interactor Systematic Name | Interactor | Interactor Systematic Name | Assay | Annotation | Action | Modification | Source | Reference | Note |
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| Complement ID | Locus ID | Gene | Species | Gene ID | Strain background | Direction | Details | Source | Reference |
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| Evidence ID | Analyze ID | Dataset | Description | Keywords | Number of Conditions | Reference |
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