A new type of cadmium (Cd) ion cell surface adsorbent was developed by integrating bacteriophage display peptide library technology with cell surface display technology. Cd2+ chelating resin served as the target molecule in screening experiments, leading to the identification of four Cd2+ -binding peptides. These peptides were introduced into Saccharomyces cerevisiae via the pYD1 plasmid using lithium acetate heat shock transformation. Adsorption efficiency tests indicated that the engineered yeasts adsorbed more Cd2+ than the control strain EBY100 when exposed to the same amount of Cd2+. Among these peptides, sequence 3-containing strain was demonstrated to have the highest Cd2+ adsorption efficiency, being 35% higher than the control strain. Additionally, when this recombinant yeast strain was immobilized using sodium alginate, the adsorption efficiency was increased by 55.7% compared to the control strain.
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| Evidence ID | Analyze ID | Gene/Complex | Systematic Name/Complex Accession | Qualifier | Gene Ontology Term ID | Gene Ontology Term | Aspect | Annotation Extension | Evidence | Method | Source | Assigned On | Reference |
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| Evidence ID | Analyze ID | Gene | Gene Systematic Name | Phenotype | Experiment Type | Experiment Type Category | Mutant Information | Strain Background | Chemical | Details | Reference |
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| Evidence ID | Analyze ID | Gene | Gene Systematic Name | Disease Ontology Term | Disease Ontology Term ID | Qualifier | Evidence | Method | Source | Assigned On | Reference |
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| Evidence ID | Analyze ID | Regulator | Regulator Systematic Name | Target | Target Systematic Name | Direction | Regulation of | Happens During | Regulator Type | Direction | Regulation Of | Happens During | Method | Evidence | Strain Background | Reference |
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| Site | Modification | Modifier | Source | Reference |
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| Evidence ID | Analyze ID | Interactor | Interactor Systematic Name | Interactor | Interactor Systematic Name | Allele | Assay | Annotation | Action | Phenotype | SGA score | P-value | Source | Reference | Note |
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| Evidence ID | Analyze ID | Interactor | Interactor Systematic Name | Interactor | Interactor Systematic Name | Assay | Annotation | Action | Modification | Source | Reference | Note |
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| Complement ID | Locus ID | Gene | Species | Gene ID | Strain background | Direction | Details | Source | Reference |
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| Evidence ID | Analyze ID | Dataset | Description | Keywords | Number of Conditions | Reference |
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| Evidence ID | Analyze ID | File | Description |
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