Patulin (PAT) is a hazardous mycotoxin that contaminates fruits and their products, causing significant economic losses. An aldo-keto reductase from Saccharomyces cerevisiae (ScAKR) was expressed in Escherichia coli in this study. The purified ScAKR converted PAT to E-ascladiol with NADPH as a cofactor. The ScAKR exhibited a strong degradation activity on PAT and the optimal degradation conditions were pH 7 and 37 °C. Molecular docking and site-specific mutagenesis indicated that the amino acids in ScAKR interacting with PAT aldehyde affected the degradation effect, and the mutation of Trp298 showed the most significant effect on the degradation rate. Furthermore, ScAKR also showed a strong degradation effect on 3-keto-deoxynivalenol, a metabolite of another mycotoxin, deoxynivalenol (DON). The findings offer new insights on the detoxification mechanism of PAT by S. cerevisiae and for the development and application of bioenzymes with broad-spectrum mycotoxin degradation properties.
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| Evidence ID | Analyze ID | Gene/Complex | Systematic Name/Complex Accession | Qualifier | Gene Ontology Term ID | Gene Ontology Term | Aspect | Annotation Extension | Evidence | Method | Source | Assigned On | Reference |
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| Evidence ID | Analyze ID | Gene | Gene Systematic Name | Phenotype | Experiment Type | Experiment Type Category | Mutant Information | Strain Background | Chemical | Details | Reference |
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| Evidence ID | Analyze ID | Gene | Gene Systematic Name | Disease Ontology Term | Disease Ontology Term ID | Qualifier | Evidence | Method | Source | Assigned On | Reference |
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| Evidence ID | Analyze ID | Regulator | Regulator Systematic Name | Target | Target Systematic Name | Direction | Regulation of | Happens During | Regulator Type | Direction | Regulation Of | Happens During | Method | Evidence | Strain Background | Reference |
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| Site | Modification | Modifier | Source | Reference |
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| Evidence ID | Analyze ID | Interactor | Interactor Systematic Name | Interactor | Interactor Systematic Name | Allele | Assay | Annotation | Action | Phenotype | SGA score | P-value | Source | Reference | Note |
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| Evidence ID | Analyze ID | Interactor | Interactor Systematic Name | Interactor | Interactor Systematic Name | Assay | Annotation | Action | Modification | Source | Reference | Note |
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| Complement ID | Locus ID | Gene | Species | Gene ID | Strain background | Direction | Details | Source | Reference |
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| Evidence ID | Analyze ID | Dataset | Description | Keywords | Number of Conditions | Reference |
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