Ginsenosides are a class of triterpenoids from the ginseng genus, with many medicinal properties. Traditionally, ginsenosides are extracted from ginseng plants to satisfy market demand; however, this approach requires substantial plant biomass and a lengthy six-year growth period before harvest. The advancement of synthetic biology allows the production of ginsenosides by engineered yeast. In this study, we combined our previously reported cultivation method with in situ extraction to enhance the production and exportation of intracellular ginsenosides by the engineered Saccharomyces cerevisiae. Remarkably, ginsenoside production reached as high as 3.4 g/L in a single shake flask, with almost 100% of ginsenosides in the organic phase. The "empty yeasts" were successfully reused 10 times in sequential cultivations. These findings are discussed in the context of cultivation intensification for natural product synthesis. Increasing the level of triterpenoid synthesis facilitates rapid development and supports the industrialization of this intriguing group of natural products.
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| Evidence ID | Analyze ID | Gene/Complex | Systematic Name/Complex Accession | Qualifier | Gene Ontology Term ID | Gene Ontology Term | Aspect | Annotation Extension | Evidence | Method | Source | Assigned On | Reference |
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| Evidence ID | Analyze ID | Gene | Gene Systematic Name | Phenotype | Experiment Type | Experiment Type Category | Mutant Information | Strain Background | Chemical | Details | Reference |
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| Evidence ID | Analyze ID | Gene | Gene Systematic Name | Disease Ontology Term | Disease Ontology Term ID | Qualifier | Evidence | Method | Source | Assigned On | Reference |
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| Evidence ID | Analyze ID | Regulator | Regulator Systematic Name | Target | Target Systematic Name | Direction | Regulation of | Happens During | Regulator Type | Direction | Regulation Of | Happens During | Method | Evidence | Strain Background | Reference |
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| Site | Modification | Modifier | Source | Reference |
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| Evidence ID | Analyze ID | Interactor | Interactor Systematic Name | Interactor | Interactor Systematic Name | Allele | Assay | Annotation | Action | Phenotype | SGA score | P-value | Source | Reference | Note |
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Increase the total number of rows showing on this page by using the pull-down located below the table, or use the page scroll at the table's top right to browse through the table's pages; use the arrows to the right of a column header to sort by that column; filter the table using the "Filter" box at the top of the table; click on the small "i" buttons located within a cell for an annotation to view further details about experiment type and any other genes involved in the interaction.
| Evidence ID | Analyze ID | Interactor | Interactor Systematic Name | Interactor | Interactor Systematic Name | Assay | Annotation | Action | Modification | Source | Reference | Note |
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| Complement ID | Locus ID | Gene | Species | Gene ID | Strain background | Direction | Details | Source | Reference |
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| Evidence ID | Analyze ID | Dataset | Description | Keywords | Number of Conditions | Reference |
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