Piceatannol, a stilbene widely found in many types of foods, shows a variety of effects on the cardiovascular system, nervous system, and skin. Its low abundance in nature impedes its development. Microbial cell factories offer a green and sustainable approach to the production of natural products. In this study, we screened two 4-coumarate:coenzyme A ligases (4CLs), nine stilbene synthases (STSs), and five 4-hydroxyphenylacetate-3-hydroxylases (4HPA3Hs) to construct multiple bioconversion routes from p-coumaric acid to piceatannol in a single Saccharomyces cerevisiae strain. Then, the supply of p-coumaric acid and malonyl-CoA was enhanced, and NADPH and FADH2 regenerations were optimized, resulting in establishment of yPi101 which produced 127 mg/L and 442 mg/L piceatannol in a flask and by fed-batch fermentation, respectively. This is the first report of the de novo production of piceatannol in a single S. cerevisiae strain and represents the highest piceatannol yield.
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| Evidence ID | Analyze ID | Gene/Complex | Systematic Name/Complex Accession | Qualifier | Gene Ontology Term ID | Gene Ontology Term | Aspect | Annotation Extension | Evidence | Method | Source | Assigned On | Reference |
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| Evidence ID | Analyze ID | Gene | Gene Systematic Name | Phenotype | Experiment Type | Experiment Type Category | Mutant Information | Strain Background | Chemical | Details | Reference |
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| Evidence ID | Analyze ID | Gene | Gene Systematic Name | Disease Ontology Term | Disease Ontology Term ID | Qualifier | Evidence | Method | Source | Assigned On | Reference |
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| Evidence ID | Analyze ID | Regulator | Regulator Systematic Name | Target | Target Systematic Name | Direction | Regulation of | Happens During | Regulator Type | Direction | Regulation Of | Happens During | Method | Evidence | Strain Background | Reference |
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| Site | Modification | Modifier | Source | Reference |
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| Evidence ID | Analyze ID | Interactor | Interactor Systematic Name | Interactor | Interactor Systematic Name | Allele | Assay | Annotation | Action | Phenotype | SGA score | P-value | Source | Reference | Note |
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| Evidence ID | Analyze ID | Interactor | Interactor Systematic Name | Interactor | Interactor Systematic Name | Assay | Annotation | Action | Modification | Source | Reference | Note |
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| Complement ID | Locus ID | Gene | Species | Gene ID | Strain background | Direction | Details | Source | Reference |
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| Evidence ID | Analyze ID | Dataset | Description | Keywords | Number of Conditions | Reference |
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