The quality formation of fermented meat products is closely linked to microorganisms, with spices potentially influencing microbial activity. There is a potential effect of spices on protease activity and myofibrillar proteins (MPs) hydrolysis during fermentation, which was explored in this study. Eugenol (EG), the main component of clove extract, was extracted, and its inhibitory effect on protease activity was found to be concentration-dependent. Ultraviolet-visible spectroscopy and fluorescence spectroscopy revealed that the absorption peak intensity of tyrosine, phenylalanine, and other residues altered. Additionally, the content of β-sheets decreased, while the proportion of other secondary structures increased. High EG concentrations significantly enhanced the solubility of MPs after protease hydrolysis while reducing the degree of hydrolysis and peptide content (P < 0.05). Tertiary structure analysis revealed that high EG concentrations reduced the hydrolysis of MPs by the protease, decreased the β-sheet content. Molecular docking and dynamics simulations showed that EG increased hydrogen bonding and decreased binding energy between protease and MPs, while the protease conformation adjusted to stabilize interactions. These findings offer insights into how spices affect protease function during meat fermentation.
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| Evidence ID | Analyze ID | Gene/Complex | Systematic Name/Complex Accession | Qualifier | Gene Ontology Term ID | Gene Ontology Term | Aspect | Annotation Extension | Evidence | Method | Source | Assigned On | Reference |
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| Evidence ID | Analyze ID | Gene | Gene Systematic Name | Phenotype | Experiment Type | Experiment Type Category | Mutant Information | Strain Background | Chemical | Details | Reference |
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| Evidence ID | Analyze ID | Gene | Gene Systematic Name | Disease Ontology Term | Disease Ontology Term ID | Qualifier | Evidence | Method | Source | Assigned On | Reference |
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| Evidence ID | Analyze ID | Regulator | Regulator Systematic Name | Target | Target Systematic Name | Direction | Regulation of | Happens During | Regulator Type | Direction | Regulation Of | Happens During | Method | Evidence | Strain Background | Reference |
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| Site | Modification | Modifier | Source | Reference |
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| Evidence ID | Analyze ID | Interactor | Interactor Systematic Name | Interactor | Interactor Systematic Name | Allele | Assay | Annotation | Action | Phenotype | SGA score | P-value | Source | Reference | Note |
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| Evidence ID | Analyze ID | Interactor | Interactor Systematic Name | Interactor | Interactor Systematic Name | Assay | Annotation | Action | Modification | Source | Reference | Note |
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| Complement ID | Locus ID | Gene | Species | Gene ID | Strain background | Direction | Details | Source | Reference |
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| Evidence ID | Analyze ID | Dataset | Description | Keywords | Number of Conditions | Reference |
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