The TRAMP (Trf4-Air2-Mtr4 polyadenylation) complex is a multiprotein assembly that targets RNA substrates for processing or degradation by the nuclear exosome. In Saccharomyces cerevisiae, the helicase activity of Mtr4 is enhanced by TRAMP assembly. Here we identify unexpected species-specific differences in the ability of Mtr4 to unwind a model RNA substrate. Unlike S. cerevisiae Mtr4, Schizosaccharomyces pombe Mtr4 retains RNA-stimulated ATPase activity, but is unable to unwind a model RNA substrate. This decoupling of ATPase and helicase activity is overcome by TRAMP formation. We further demonstrate that activation of helicase activity is accomplished by unique interactions with multiple regions of the intrinsically disordered N-terminus of the poly(A) polymerase, Cid14 (the S. pombe homologue of S. cerevisiae Trf4). Finally, we propose a model where Mtr4 adaptor complexes regulate unwinding activity by coordinating interdomain interactions within the helicase core.
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| Evidence ID | Analyze ID | Gene/Complex | Systematic Name/Complex Accession | Qualifier | Gene Ontology Term ID | Gene Ontology Term | Aspect | Annotation Extension | Evidence | Method | Source | Assigned On | Reference |
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| Evidence ID | Analyze ID | Gene | Gene Systematic Name | Phenotype | Experiment Type | Experiment Type Category | Mutant Information | Strain Background | Chemical | Details | Reference |
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| Evidence ID | Analyze ID | Gene | Gene Systematic Name | Disease Ontology Term | Disease Ontology Term ID | Qualifier | Evidence | Method | Source | Assigned On | Reference |
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| Evidence ID | Analyze ID | Regulator | Regulator Systematic Name | Target | Target Systematic Name | Direction | Regulation of | Happens During | Regulator Type | Direction | Regulation Of | Happens During | Method | Evidence | Strain Background | Reference |
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| Site | Modification | Modifier | Source | Reference |
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| Evidence ID | Analyze ID | Interactor | Interactor Systematic Name | Interactor | Interactor Systematic Name | Allele | Assay | Annotation | Action | Phenotype | SGA score | P-value | Source | Reference | Note |
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| Evidence ID | Analyze ID | Interactor | Interactor Systematic Name | Interactor | Interactor Systematic Name | Assay | Annotation | Action | Modification | Source | Reference | Note |
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| Complement ID | Locus ID | Gene | Species | Gene ID | Strain background | Direction | Details | Source | Reference |
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| Evidence ID | Analyze ID | Dataset | Description | Keywords | Number of Conditions | Reference |
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