Peroxisomes are dynamic organelles that contribute to diverse metabolic functions, including β-oxidation, photorespiration, and phytohormone biosynthesis. Peroxisomes import proteins from the cytosol through the action of peroxins (PEX proteins), many of which are conserved among fungi, plants, and animals. An apparent exception is Pex8, which is essential for lumenal protein import in several yeast species but has not been reported outside of fungi. Here, we identified an uncharacterized Arabidopsis thaliana protein with predicted structural similarity to Saccharomyces cerevisiae Pex8. Like yeast Pex8, Arabidopsis PEX8 is primarily composed of predicted HEAT repeats and has two predicted peroxisome-targeting signals. pex8 insertional and frameshift mutations were lethal, whereas expressing an artificial microRNA targeting PEX8 impaired lumenal protein import into peroxisomes and conferred physiological defects indicative of peroxisome dysfunction. Fluorescent reporters fused to the N terminus of PEX8 localized within peroxisomes in puncta associated with peroxisomal membranes. Our data show that Arabidopsis PEX8 is functionally equivalent to yeast Pex8, revealing the conservation of peroxisomal protein import machinery across eukaryotes and raising the intriguing possibility that other "yeast-specific" peroxins have eluded discovery in plants and mammals because of low primary sequence conservation.
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Evidence ID | Analyze ID | Gene/Complex | Systematic Name/Complex Accession | Qualifier | Gene Ontology Term ID | Gene Ontology Term | Aspect | Annotation Extension | Evidence | Method | Source | Assigned On | Reference |
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Evidence ID | Analyze ID | Gene | Gene Systematic Name | Phenotype | Experiment Type | Experiment Type Category | Mutant Information | Strain Background | Chemical | Details | Reference |
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Evidence ID | Analyze ID | Gene | Gene Systematic Name | Disease Ontology Term | Disease Ontology Term ID | Qualifier | Evidence | Method | Source | Assigned On | Reference |
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Evidence ID | Analyze ID | Regulator | Regulator Systematic Name | Target | Target Systematic Name | Direction | Regulation of | Happens During | Regulator Type | Direction | Regulation Of | Happens During | Method | Evidence | Strain Background | Reference |
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Site | Modification | Modifier | Source | Reference |
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Evidence ID | Analyze ID | Interactor | Interactor Systematic Name | Interactor | Interactor Systematic Name | Allele | Assay | Annotation | Action | Phenotype | SGA score | P-value | Source | Reference | Note |
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Evidence ID | Analyze ID | Interactor | Interactor Systematic Name | Interactor | Interactor Systematic Name | Assay | Annotation | Action | Modification | Source | Reference | Note |
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Complement ID | Locus ID | Gene | Species | Gene ID | Strain background | Direction | Details | Source | Reference |
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Evidence ID | Analyze ID | Dataset | Description | Keywords | Number of Conditions | Reference |
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