Although tea polyphenols have antiaging potential, the molecular interplay between black tea components and cellular longevity remains unclear. This study has pioneered a dual approach combining Saccharomyces cerevisiae lifespan assays with comprehensive transcriptomics to dissect how black tea aqueous extract (BTAE) extends the chronological lifespan (CLS). Remarkably, BTAE induced a dose-dependent lifespan extension (34.6% maximum at 40 μg/mL), coupled with a 62% reduction in reactive oxygen species (ROS) accumulation and enhanced superoxide dismutase/catalase/ascorbate peroxidase (SOD/CAT/APX) enzyme activities-hallmarks of antioxidant system activation. Transcriptomic profiling revealed the action mechanism of BTAE: (1) upregulation of oxidative defense arsenals (SOD1/2, CTA1, and CTT1) and mitochondrial efficiency genes (COX10), and (2) strategic downregulation of apoptosis triggers (NMA111 and AIF1) and aging accelerators (MTH1 and HSPs). Additionally, we have identified novel targets including the OLE1-mediated membrane protection pathway and IRE1-dependent proteostatic regulation as key longevity switches. These findings establish BTAE as a multi-target anti-aging modulator and offer a roadmap for translating yeast discovery into mammalian aging interventions.
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| Evidence ID | Analyze ID | Gene/Complex | Systematic Name/Complex Accession | Qualifier | Gene Ontology Term ID | Gene Ontology Term | Aspect | Annotation Extension | Evidence | Method | Source | Assigned On | Reference |
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| Evidence ID | Analyze ID | Gene | Gene Systematic Name | Phenotype | Experiment Type | Experiment Type Category | Mutant Information | Strain Background | Chemical | Details | Reference |
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| Evidence ID | Analyze ID | Gene | Gene Systematic Name | Disease Ontology Term | Disease Ontology Term ID | Qualifier | Evidence | Method | Source | Assigned On | Reference |
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| Evidence ID | Analyze ID | Regulator | Regulator Systematic Name | Target | Target Systematic Name | Direction | Regulation of | Happens During | Regulator Type | Direction | Regulation Of | Happens During | Method | Evidence | Strain Background | Reference |
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| Site | Modification | Modifier | Source | Reference |
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| Evidence ID | Analyze ID | Interactor | Interactor Systematic Name | Interactor | Interactor Systematic Name | Allele | Assay | Annotation | Action | Phenotype | SGA score | P-value | Source | Reference | Note |
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| Evidence ID | Analyze ID | Interactor | Interactor Systematic Name | Interactor | Interactor Systematic Name | Assay | Annotation | Action | Modification | Source | Reference | Note |
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| Complement ID | Locus ID | Gene | Species | Gene ID | Strain background | Direction | Details | Source | Reference |
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| Evidence ID | Analyze ID | Dataset | Description | Keywords | Number of Conditions | Reference |
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