Quorum sensing (QS) is a cell-to-cell signaling system that takes place at a key concentration (quorum) of signal molecules and via a peculiar signaling pathway. Both bacteria and yeasts possess QS mechanisms, mediated by specific molecules (farnesol, tyrosol, 2-phenylethanol, tryptophol) in yeasts, and N-acylhomoserine lactones (AHLs) and modified oligopeptides in bacteria. Here, we report the first chemical evidence of bacterial QS activity in yeast Saccharomyces cerevisiae (OS3 and V5 strains) by UPLC-MS/MS identification of N-octanoyl- and N-decanoyl-L-homoserine lactones in cell-free culture media extracts. The AHLs' presence was unexpected, as they are produced exclusively by bacteria. Tyrosol, a yeast signal molecule, was identified and quantified by NMR analysis. Metataxonomic analysis suggested the existence inside S. cerevisiae cells of bacteria, including Firmicutes, Bacteroidota, and Proteobacteria. Our study paves the way for investigations into bacterial detection within S. cerevisiae cells and their role in biotechnological performance in the food fermentation fields.
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| Evidence ID | Analyze ID | Gene/Complex | Systematic Name/Complex Accession | Qualifier | Gene Ontology Term ID | Gene Ontology Term | Aspect | Annotation Extension | Evidence | Method | Source | Assigned On | Reference |
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| Evidence ID | Analyze ID | Gene | Gene Systematic Name | Phenotype | Experiment Type | Experiment Type Category | Mutant Information | Strain Background | Chemical | Details | Reference |
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| Evidence ID | Analyze ID | Gene | Gene Systematic Name | Disease Ontology Term | Disease Ontology Term ID | Qualifier | Evidence | Method | Source | Assigned On | Reference |
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| Evidence ID | Analyze ID | Regulator | Regulator Systematic Name | Target | Target Systematic Name | Direction | Regulation of | Happens During | Regulator Type | Direction | Regulation Of | Happens During | Method | Evidence | Strain Background | Reference |
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| Site | Modification | Modifier | Source | Reference |
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| Evidence ID | Analyze ID | Interactor | Interactor Systematic Name | Interactor | Interactor Systematic Name | Allele | Assay | Annotation | Action | Phenotype | SGA score | P-value | Source | Reference | Note |
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| Evidence ID | Analyze ID | Interactor | Interactor Systematic Name | Interactor | Interactor Systematic Name | Assay | Annotation | Action | Modification | Source | Reference | Note |
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| Complement ID | Locus ID | Gene | Species | Gene ID | Strain background | Direction | Details | Source | Reference |
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| Evidence ID | Analyze ID | Dataset | Description | Keywords | Number of Conditions | Reference |
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| Evidence ID | Analyze ID | File | Description |
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