CMAC (7-amino-4-chloromethylcoumarin) staining is a valuable tool for visualizing acidic organelles, including vacuoles, in yeast cells. By selectively accumulating in acidic compartments, CMAC allows specific labeling and visualization of vacuoles in living or fixed cells, aiding in the investigation of vacuole dynamics, morphology, and distribution under various conditions. This staining method provides a clear contrast against cellular autofluorescence, enhancing imaging clarity. It can be combined with automated imaging capture for faster throughput and analysis.Characterizing vacuole phenotypes using CMAC staining contributes to our understanding of cellular homeostasis and disease mechanisms in yeast. It has applications in studying fundamental cellular processes, including endocytic trafficking and autophagy, as well as in drug screening assays, and can be used in yeast models of disease to offer insights into potential therapeutic targets for diseases affecting the lysosome, including neurodegenerative diseases.
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| Evidence ID | Analyze ID | Gene/Complex | Systematic Name/Complex Accession | Qualifier | Gene Ontology Term ID | Gene Ontology Term | Aspect | Annotation Extension | Evidence | Method | Source | Assigned On | Reference |
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| Evidence ID | Analyze ID | Gene | Gene Systematic Name | Phenotype | Experiment Type | Experiment Type Category | Mutant Information | Strain Background | Chemical | Details | Reference |
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| Evidence ID | Analyze ID | Gene | Gene Systematic Name | Disease Ontology Term | Disease Ontology Term ID | Qualifier | Evidence | Method | Source | Assigned On | Reference |
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| Evidence ID | Analyze ID | Regulator | Regulator Systematic Name | Target | Target Systematic Name | Direction | Regulation of | Happens During | Regulator Type | Direction | Regulation Of | Happens During | Method | Evidence | Strain Background | Reference |
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| Site | Modification | Modifier | Source | Reference |
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| Evidence ID | Analyze ID | Interactor | Interactor Systematic Name | Interactor | Interactor Systematic Name | Allele | Assay | Annotation | Action | Phenotype | SGA score | P-value | Source | Reference | Note |
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Increase the total number of rows showing on this page by using the pull-down located below the table, or use the page scroll at the table's top right to browse through the table's pages; use the arrows to the right of a column header to sort by that column; filter the table using the "Filter" box at the top of the table; click on the small "i" buttons located within a cell for an annotation to view further details about experiment type and any other genes involved in the interaction.
| Evidence ID | Analyze ID | Interactor | Interactor Systematic Name | Interactor | Interactor Systematic Name | Assay | Annotation | Action | Modification | Source | Reference | Note |
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| Complement ID | Locus ID | Gene | Species | Gene ID | Strain background | Direction | Details | Source | Reference |
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| Evidence ID | Analyze ID | Dataset | Description | Keywords | Number of Conditions | Reference |
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