The cohesin loader complex, consisting in Scc2 and Scc4 subunits, allows cohesin access to chromosomes, and therefore, it is required for cohesin's essential roles in many structural and functional aspects of chromosomes such as sister chromatid cohesion, genome organization, gene transcription, and DNA repair. The cohesin loader interacts with the RSC chromatin remodeling complex directing cohesin loading to nucleosome-free regions. In addition to stimulate cohesin loading and define the cohesin loading sites along the genome, the cohesin loader plays a role in nucleosome positioning at gene promoters, suggesting an interplay between cohesin loading and chromatin remodeling. Here, we describe an in vitro assay to assess the ability of the cohesin loader to stimulate RSC nucleosome sliding activity.
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| Evidence ID | Analyze ID | Gene/Complex | Systematic Name/Complex Accession | Qualifier | Gene Ontology Term ID | Gene Ontology Term | Aspect | Annotation Extension | Evidence | Method | Source | Assigned On | Reference |
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| Evidence ID | Analyze ID | Gene | Gene Systematic Name | Phenotype | Experiment Type | Experiment Type Category | Mutant Information | Strain Background | Chemical | Details | Reference |
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| Evidence ID | Analyze ID | Gene | Gene Systematic Name | Disease Ontology Term | Disease Ontology Term ID | Qualifier | Evidence | Method | Source | Assigned On | Reference |
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| Evidence ID | Analyze ID | Regulator | Regulator Systematic Name | Target | Target Systematic Name | Direction | Regulation of | Happens During | Regulator Type | Direction | Regulation Of | Happens During | Method | Evidence | Strain Background | Reference |
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| Site | Modification | Modifier | Source | Reference |
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| Evidence ID | Analyze ID | Interactor | Interactor Systematic Name | Interactor | Interactor Systematic Name | Allele | Assay | Annotation | Action | Phenotype | SGA score | P-value | Source | Reference | Note |
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| Evidence ID | Analyze ID | Interactor | Interactor Systematic Name | Interactor | Interactor Systematic Name | Assay | Annotation | Action | Modification | Source | Reference | Note |
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| Complement ID | Locus ID | Gene | Species | Gene ID | Strain background | Direction | Details | Source | Reference |
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| Evidence ID | Analyze ID | Dataset | Description | Keywords | Number of Conditions | Reference |
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