Acetic acid, a major byproduct of Saccharomyces cerevisiae alcoholic fermentation, hinder fermentation efficiency and wine quality. This study improved wine yeast strains through ARTP mutagenesis, resulting in mutant DC-3 with enhanced fermentation performance and reduced acetic acid production. Analysis of DC-3 showed improved oenological properties, including a 25.84 % decrease in acetic acid production compared to wild-type strain RV002 in synthetic grape juice. The study identified a regulatory mechanism in DC-3 that reduces acetate production by suppressing pyruvate decarboxylase, enhancing alcohol dehydrogenase, and inhibiting acetaldehyde dehydrogenase activities, while redirecting metabolism towards glycerol biosynthesis. Transcriptomic analysis confirmed this metabolic shift. DC-3 consistently produced lower acetic acid levels and exhibited a unique volatile profile with higher alcohols and lower esters, specifically reducing acetate esters compared to RV002 in Cabernet Sauvignon wine. This study introduces a novel approach to breeding low-acetate-producing S. cerevisiae strains in Cabernet Sauvignon wine fermentation using ARTP mutagenesis. It lays the groundwork for enhancing wine fermentation processes.
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| Evidence ID | Analyze ID | Gene/Complex | Systematic Name/Complex Accession | Qualifier | Gene Ontology Term ID | Gene Ontology Term | Aspect | Annotation Extension | Evidence | Method | Source | Assigned On | Reference |
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| Evidence ID | Analyze ID | Gene | Gene Systematic Name | Phenotype | Experiment Type | Experiment Type Category | Mutant Information | Strain Background | Chemical | Details | Reference |
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| Evidence ID | Analyze ID | Gene | Gene Systematic Name | Disease Ontology Term | Disease Ontology Term ID | Qualifier | Evidence | Method | Source | Assigned On | Reference |
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| Evidence ID | Analyze ID | Regulator | Regulator Systematic Name | Target | Target Systematic Name | Direction | Regulation of | Happens During | Regulator Type | Direction | Regulation Of | Happens During | Method | Evidence | Strain Background | Reference |
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| Site | Modification | Modifier | Source | Reference |
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| Evidence ID | Analyze ID | Interactor | Interactor Systematic Name | Interactor | Interactor Systematic Name | Allele | Assay | Annotation | Action | Phenotype | SGA score | P-value | Source | Reference | Note |
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| Evidence ID | Analyze ID | Interactor | Interactor Systematic Name | Interactor | Interactor Systematic Name | Assay | Annotation | Action | Modification | Source | Reference | Note |
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| Complement ID | Locus ID | Gene | Species | Gene ID | Strain background | Direction | Details | Source | Reference |
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| Evidence ID | Analyze ID | Dataset | Description | Keywords | Number of Conditions | Reference |
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