In budding yeast, Dmc1's recombinogenic activity is controlled by the meiosis-specific heterodimer Mei5-Sae3. Mei5-Sae3 is required for assembly of Dmc1 at sites of meiotic DNA double-stranded breaks. Here, we report Mei5-Sae3 can stabilize Dmc1 filaments in both the active and inactive allosteric conformations depending on the nucleotide cofactor supporting filament formation. Mei5-Sae3 specifically stabilizes the active filament form without inhibiting ATP hydrolysis, in contrast to high concentrations of calcium, AMP-PNP, and the E157D mutation in Dmc1, each of which promotes Dmc1 filament stability by processes that include blocks to ATP hydrolysis. Mei5-Sae3 increases Dmc1 ATP hydrolysis by a mechanism that could be a cause of active filament stabilization or a secondary and inconsequential effect of active filament stabilization. Mei5-Sae3 can also stabilize filaments in the inactive conformation with ADP as a cofactor. These results show that Mei5-Sae3's filament stabilization activity does not fully depend on alteration of the hydrolytic cycle. We also show Dmc1-E157D, a gain-of-function protein that bypasses the requirement for Mei5-Sae3 in vivo, is defective in ATPase activity and stabilizes the active form of Dmc1 filaments as predicted by previous observations. Hence, Dmc1's homology search and strand exchange activities do not depend on its ability to hydrolyze ATP.
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| Evidence ID | Analyze ID | Gene/Complex | Systematic Name/Complex Accession | Qualifier | Gene Ontology Term ID | Gene Ontology Term | Aspect | Annotation Extension | Evidence | Method | Source | Assigned On | Reference |
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| Evidence ID | Analyze ID | Gene | Gene Systematic Name | Phenotype | Experiment Type | Experiment Type Category | Mutant Information | Strain Background | Chemical | Details | Reference |
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| Evidence ID | Analyze ID | Gene | Gene Systematic Name | Disease Ontology Term | Disease Ontology Term ID | Qualifier | Evidence | Method | Source | Assigned On | Reference |
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| Evidence ID | Analyze ID | Regulator | Regulator Systematic Name | Target | Target Systematic Name | Direction | Regulation of | Happens During | Regulator Type | Direction | Regulation Of | Happens During | Method | Evidence | Strain Background | Reference |
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| Site | Modification | Modifier | Source | Reference |
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| Evidence ID | Analyze ID | Interactor | Interactor Systematic Name | Interactor | Interactor Systematic Name | Allele | Assay | Annotation | Action | Phenotype | SGA score | P-value | Source | Reference | Note |
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| Evidence ID | Analyze ID | Interactor | Interactor Systematic Name | Interactor | Interactor Systematic Name | Assay | Annotation | Action | Modification | Source | Reference | Note |
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| Complement ID | Locus ID | Gene | Species | Gene ID | Strain background | Direction | Details | Source | Reference |
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| Evidence ID | Analyze ID | Dataset | Description | Keywords | Number of Conditions | Reference |
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| Evidence ID | Analyze ID | File | Description |
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