β-elemene, a clinically approved antineoplastic agent, is isolated from Curcuma wenyujin at low abundance. Germacrene A is its direct precursor. To overcome the supply bottleneck, we systematically rewired the mevalonate pathway of Saccharomyces cerevisiae to expand the universal C15 precursor pool. Among 19 screened germacrene A synthases, DeGAS from Daldinia eschscholtzii yielded 399.8 mg/L. Fusion of DeGAS to farnesyl pyrophosphate synthase via the rigid (EAAAK)₂ linker increased the titer to 492 mg/L, and triplication of the ERG20-DeGAS cassette further raised it to 985.5 mg/L. In a 5-L fed-batch bioreactor, germacrene A reached 19.82 g/L, a 20-fold gain over shake-flask cultures, and comparable to the highest titers previously reported in S. cerevisiae. This work provides a scalable microbial route to β-elemene and demonstrates a general strategy for sesquiterpene overproduction.
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| Evidence ID | Analyze ID | Gene/Complex | Systematic Name/Complex Accession | Qualifier | Gene Ontology Term ID | Gene Ontology Term | Aspect | Annotation Extension | Evidence | Method | Source | Assigned On | Reference |
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| Evidence ID | Analyze ID | Gene | Gene Systematic Name | Phenotype | Experiment Type | Experiment Type Category | Mutant Information | Strain Background | Chemical | Details | Reference |
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| Evidence ID | Analyze ID | Gene | Gene Systematic Name | Disease Ontology Term | Disease Ontology Term ID | Qualifier | Evidence | Method | Source | Assigned On | Reference |
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| Evidence ID | Analyze ID | Regulator | Regulator Systematic Name | Target | Target Systematic Name | Direction | Regulation of | Happens During | Regulator Type | Direction | Regulation Of | Happens During | Method | Evidence | Strain Background | Reference |
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| Site | Modification | Modifier | Source | Reference |
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| Evidence ID | Analyze ID | Interactor | Interactor Systematic Name | Interactor | Interactor Systematic Name | Allele | Assay | Annotation | Action | Phenotype | SGA score | P-value | Source | Reference | Note |
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| Evidence ID | Analyze ID | Interactor | Interactor Systematic Name | Interactor | Interactor Systematic Name | Assay | Annotation | Action | Modification | Source | Reference | Note |
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| Complement ID | Locus ID | Gene | Species | Gene ID | Strain background | Direction | Details | Source | Reference |
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| Evidence ID | Analyze ID | Dataset | Description | Keywords | Number of Conditions | Reference |
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