A co-inoculation strategy using Saccharomyces cerevisiae VW followed by the indigenous Oenococcus oeni strain MG-1 after 48 h (VM-48) was found to significantly reduce fermentation duration and modulate the organoleptic profile of Chardonnay wine. To analyze the chemical basis of their flavor characteristics, the key odorants in the co-inoculated wines were identified using sensory descriptive analysis, omission, and addition tests. Furthermore, their interaction effects were explored using threshold and σ-τ plot methodology. Results show that the co-inoculation protocol of VM-48 achieved the highest malate degradation ratio (96.8 %), lactate yield (1.67 g/L), and volatiles accumulation level (49.6 g/L). Isoamyl acetate, ethyl caprate, ethyl octanoate, ethyl butyrate, 2-methylbutanoic acid, β-damascenone, and phenylacetaldehyde are the key odorants in VM-48 wine, which ultimately contribute to its intense tropical fruit, citrus, stone fruit, and floral notes. With the exception of the hyper-addition effects between β-damascenone or isoamyl acetate and ethyl butyrate, the aroma perception of VM-48 wine was further enhanced through partial addition effects between the key aroma-active composition with similar chemical structures or concentrations.
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| Evidence ID | Analyze ID | Gene/Complex | Systematic Name/Complex Accession | Qualifier | Gene Ontology Term ID | Gene Ontology Term | Aspect | Annotation Extension | Evidence | Method | Source | Assigned On | Reference |
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| Evidence ID | Analyze ID | Gene | Gene Systematic Name | Phenotype | Experiment Type | Experiment Type Category | Mutant Information | Strain Background | Chemical | Details | Reference |
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| Evidence ID | Analyze ID | Gene | Gene Systematic Name | Disease Ontology Term | Disease Ontology Term ID | Qualifier | Evidence | Method | Source | Assigned On | Reference |
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| Evidence ID | Analyze ID | Regulator | Regulator Systematic Name | Target | Target Systematic Name | Direction | Regulation of | Happens During | Regulator Type | Direction | Regulation Of | Happens During | Method | Evidence | Strain Background | Reference |
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| Site | Modification | Modifier | Source | Reference |
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| Evidence ID | Analyze ID | Interactor | Interactor Systematic Name | Interactor | Interactor Systematic Name | Allele | Assay | Annotation | Action | Phenotype | SGA score | P-value | Source | Reference | Note |
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| Evidence ID | Analyze ID | Interactor | Interactor Systematic Name | Interactor | Interactor Systematic Name | Assay | Annotation | Action | Modification | Source | Reference | Note |
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| Complement ID | Locus ID | Gene | Species | Gene ID | Strain background | Direction | Details | Source | Reference |
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| Evidence ID | Analyze ID | Dataset | Description | Keywords | Number of Conditions | Reference |
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