RNA oxidation is an important yet understudied process, partly because methods to localize oxidized residues in RNA are lacking. We introduce OAbSeq, a deep-sequencing approach that maps oxidized sites with high sensitivity by exploiting aniline-induced strand scission at noncanonical nucleosides to generate unique ligation-competent fragments utilized for library preparation. Applied to yeast RNA, OAbSeq detects widespread signals predominating at purines, especially at guanosines. Exogenous oxidation increased signal intensity but preserved the guanosine-dominated pattern. Parallel quantification of 8-oxoguanosine (oxo8G) and abasic sites revealed that abasic sites are more abundant than oxo8G following oxidative treatment in vitro and under physiological conditions. These data support a model in which guanosine oxidation proceeds via transient oxo8G yielding abasic sites that can be mapped at nucleotide resolution by OAbSeq. Our findings also suggest abasic sites may be a more informative marker of RNA oxidative damage than oxo8G, facilitating studies of RNA oxidation dynamics in cells.
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| Evidence ID | Analyze ID | Gene/Complex | Systematic Name/Complex Accession | Qualifier | Gene Ontology Term ID | Gene Ontology Term | Aspect | Annotation Extension | Evidence | Method | Source | Assigned On | Reference |
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| Evidence ID | Analyze ID | Gene | Gene Systematic Name | Phenotype | Experiment Type | Experiment Type Category | Mutant Information | Strain Background | Chemical | Details | Reference |
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| Evidence ID | Analyze ID | Gene | Gene Systematic Name | Disease Ontology Term | Disease Ontology Term ID | Qualifier | Evidence | Method | Source | Assigned On | Reference |
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| Evidence ID | Analyze ID | Regulator | Regulator Systematic Name | Target | Target Systematic Name | Direction | Regulation of | Happens During | Regulator Type | Direction | Regulation Of | Happens During | Method | Evidence | Strain Background | Reference |
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| Site | Modification | Modifier | Source | Reference |
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| Evidence ID | Analyze ID | Interactor | Interactor Systematic Name | Interactor | Interactor Systematic Name | Allele | Assay | Annotation | Action | Phenotype | SGA score | P-value | Source | Reference | Note |
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| Evidence ID | Analyze ID | Interactor | Interactor Systematic Name | Interactor | Interactor Systematic Name | Assay | Annotation | Action | Modification | Source | Reference | Note |
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| Complement ID | Locus ID | Gene | Species | Gene ID | Strain background | Direction | Details | Source | Reference |
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| Evidence ID | Analyze ID | Dataset | Description | Keywords | Number of Conditions | Reference |
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| Evidence ID | Analyze ID | File | Description |
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