In recent years, the yeast cell wall has gained considerable attention due to its role in stress tolerance, pathogenicity, and its high content of functional polysaccharides relevant to food and feed applications. This study introduces an improved analytical method for simultaneously quantifying β-glucan, mannoprotein, glycogen, and trehalose in both crude yeast cells and yeast β-glucan isolates. It combines acid hydrolysis of cell wall polysaccharides into monosaccharides with chromatographic sugar analysis, and includes an enzymatic hydrolysis step for glycogen and trehalose determination. Results obtained using this approach are compared with those from three established analytical methods, revealing differences in the β-glucan content of up to 37 %, depending on the analytical method used. The new analytical protocol proves to be more robust and more accurate than the existing methods for β-glucan quantification, in addition to providing valuable information on the presence of multiple cell wall polysaccharides and reserve carbohydrates.
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| Evidence ID | Analyze ID | Gene/Complex | Systematic Name/Complex Accession | Qualifier | Gene Ontology Term ID | Gene Ontology Term | Aspect | Annotation Extension | Evidence | Method | Source | Assigned On | Reference |
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| Evidence ID | Analyze ID | Gene | Gene Systematic Name | Phenotype | Experiment Type | Experiment Type Category | Mutant Information | Strain Background | Chemical | Details | Reference |
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| Evidence ID | Analyze ID | Gene | Gene Systematic Name | Disease Ontology Term | Disease Ontology Term ID | Qualifier | Evidence | Method | Source | Assigned On | Reference |
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| Evidence ID | Analyze ID | Regulator | Regulator Systematic Name | Target | Target Systematic Name | Direction | Regulation of | Happens During | Regulator Type | Direction | Regulation Of | Happens During | Method | Evidence | Strain Background | Reference |
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| Site | Modification | Modifier | Source | Reference |
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| Evidence ID | Analyze ID | Interactor | Interactor Systematic Name | Interactor | Interactor Systematic Name | Allele | Assay | Annotation | Action | Phenotype | SGA score | P-value | Source | Reference | Note |
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| Evidence ID | Analyze ID | Interactor | Interactor Systematic Name | Interactor | Interactor Systematic Name | Assay | Annotation | Action | Modification | Source | Reference | Note |
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| Complement ID | Locus ID | Gene | Species | Gene ID | Strain background | Direction | Details | Source | Reference |
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| Evidence ID | Analyze ID | Dataset | Description | Keywords | Number of Conditions | Reference |
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