Our previous research suggested that the co-cultivation of Escherichia coli and Saccharomyces cerevisiae could enhance the oxidative tolerance of yeast, and identified hexadecanoic acid as a key metabolic regulator. Nevertheless, the precise regulatory mechanisms remain unclear. The objective of this work is to elucidate the mechanism through which hexadecanoic acid enhances the oxidative tolerance of S. cerevisiae. Results suggested that addition of hexadecanoic acid could significantly reduce the reactive oxygen species (ROS) level by 25.78 % and contribute to maintaining the structural integrity of S. cerevisiae. Hexadecanoic acid reduced ROS production through inhibiting the activation of the Ca²⁺ signaling pathway. On the other hand, due to the decreased generation of intracellular ROS, hexadecanoic acid also down-regulated the antioxidant defense capability of S. cerevisiae, suggesting the non-necessity of excessive activation of the antioxidant system. Transcriptomic analysis revealed hexadecanoic acid treatment associated 27 up-regulated and 30 down-regulated genes. Based on the transcriptomics analysis results and previous findings, the MF(α)2 was chosen as a target gene to be clarified. Further construction of the MF(α)2 knockout strain demonstrated that the oxidative tolerance of ΔMF(α)2 strain significantly decreased. In light of these findings, it can be inferred that hexadecanoic acid may promote the MF(α)2 gene expression, thereby delaying the cell cycle and enhancing yeast oxidative tolerance. Results presented in this work would contribute to the understanding of the regulatory mechanisms of hexadecanoic acid on the oxidative tolerance of S. cerevisiae, and would also offer insights into the potential for manual intervention to regulate the oxidative tolerance of strains.

• PMID: 41344776
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Journal Article

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Liu H, Hou S, Wang S, Ji Y, Yu C, Li H

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