Background: Kestoses, short-chain fructooligosaccharides (FOSs), are valued for their intestinal, immunomodulatory, sweetness, and prebiotic properties compared with longer-chain FOSs. Although invertases are widely used for kestose production, most enzymes generate mixed FOS products. This study investigates a GH32 invertase from Saccharomyces cerevisiae (ScINV), heterologously expressed in Escherichia coli, with an emphasis on its product selectivity for kestose production.
Results: Under optimized conditions (600 g L-1 initial sucrose concentration, 1.0 μmol L-1 enzyme, pH 6.0, and 45 °C), ScINV produced 54.1 g L-1 kestoses after 24 h, corresponding to a yield of 9.0% and a purity of 7.5%. Purification by ligand-exchange chromatography using XA2004-30-Ca resin increased kestose purity to 19.4% with complete recovery, whereas higher-resolution fractionation achieved 100% purity with a reduced recovery of 14.9%.
Conclusion: ScINV exhibits selective kestose production without formation of higher-degree FOSs. Although partial peak overlap limits single-step recovery, purification using XA2004-30-Ca resin provides a promising basis for scale-up. Coupling this approach with continuous simulated moving bed technology may improve process efficiency and industrial feasibility. © 2026 Society of Chemical Industry.
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| Evidence ID | Analyze ID | Gene/Complex | Systematic Name/Complex Accession | Qualifier | Gene Ontology Term ID | Gene Ontology Term | Aspect | Annotation Extension | Evidence | Method | Source | Assigned On | Reference |
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| Evidence ID | Analyze ID | Gene | Gene Systematic Name | Phenotype | Experiment Type | Experiment Type Category | Mutant Information | Strain Background | Chemical | Details | Reference |
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| Evidence ID | Analyze ID | Gene | Gene Systematic Name | Disease Ontology Term | Disease Ontology Term ID | Qualifier | Evidence | Method | Source | Assigned On | Reference |
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| Evidence ID | Analyze ID | Regulator | Regulator Systematic Name | Target | Target Systematic Name | Direction | Regulation of | Happens During | Regulator Type | Direction | Regulation Of | Happens During | Method | Evidence | Strain Background | Reference |
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| Site | Modification | Modifier | Source | Reference |
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| Evidence ID | Analyze ID | Interactor | Interactor Systematic Name | Interactor | Interactor Systematic Name | Allele | Assay | Annotation | Action | Phenotype | SGA score | P-value | Source | Reference | Note |
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| Evidence ID | Analyze ID | Interactor | Interactor Systematic Name | Interactor | Interactor Systematic Name | Assay | Annotation | Action | Modification | Source | Reference | Note |
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| Complement ID | Locus ID | Gene | Species | Gene ID | Strain background | Direction | Details | Source | Reference |
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| Evidence ID | Analyze ID | Dataset | Description | Keywords | Number of Conditions | Reference |
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