In fungi, spores represent a highly resilient stage of the life cycle, characterized by low metabolic activity that confers resistance to xenobiotics. However, as soon as spores start germination, they become vulnerable to low-molecular-weight toxins. We hypothesized that during sporulation fungi presynthesize spore-specific drug-efflux transporters to mitigate this vulnerability. To test this hypothesis, we compared the repertoire of ABC- and MFS- transporters involved in multidrug resistance (MDR) between spores and proliferating cells of yeast Saccharomyces cerevisiae. Using a set of strains in which MDR-transporters are tagged with a GFP, we showed that in spores the major efflux pump is MFS transporter Flr1p, whereas in proliferating vegetative cells it is Pdr5p. In the presence of xenobiotics, deletion of the FLR1 gene reduced the growth rate of microcolonies originating from spores but did not affect growth from vegetative cells. We propose that Pdr5p's basal ATPase activity may be disadvantageous for spores, as it could be detrimental during prolonged dormancy.
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| Evidence ID | Analyze ID | Gene/Complex | Systematic Name/Complex Accession | Qualifier | Gene Ontology Term ID | Gene Ontology Term | Aspect | Annotation Extension | Evidence | Method | Source | Assigned On | Reference |
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| Evidence ID | Analyze ID | Gene | Gene Systematic Name | Phenotype | Experiment Type | Experiment Type Category | Mutant Information | Strain Background | Chemical | Details | Reference |
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| Evidence ID | Analyze ID | Gene | Gene Systematic Name | Disease Ontology Term | Disease Ontology Term ID | Qualifier | Evidence | Method | Source | Assigned On | Reference |
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| Evidence ID | Analyze ID | Regulator | Regulator Systematic Name | Target | Target Systematic Name | Direction | Regulation of | Happens During | Regulator Type | Direction | Regulation Of | Happens During | Method | Evidence | Strain Background | Reference |
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| Site | Modification | Modifier | Source | Reference |
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| Evidence ID | Analyze ID | Interactor | Interactor Systematic Name | Interactor | Interactor Systematic Name | Allele | Assay | Annotation | Action | Phenotype | SGA score | P-value | Source | Reference | Note |
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| Evidence ID | Analyze ID | Interactor | Interactor Systematic Name | Interactor | Interactor Systematic Name | Assay | Annotation | Action | Modification | Source | Reference | Note |
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| Complement ID | Locus ID | Gene | Species | Gene ID | Strain background | Direction | Details | Source | Reference |
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| Evidence ID | Analyze ID | Dataset | Description | Keywords | Number of Conditions | Reference |
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