Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided.
126 entries for 44 phenotypesIncrease the total number of rows showing on this page using the pull-down located below the table, or use the page scroll at the table's top right to browse through the table's pages; use the arrows to the right of a column header to sort by that column; filter the table using the "Filter" box at the top of the table; click on the small "i" buttons located within a cell for an annotation to view further details.
| Gene | Phenotype | Experiment Type | Mutant Information | Strain Background | Chemical | Details | Reference |
|---|---|---|---|---|---|---|---|
| VPS30 | autophagy: absent | classical genetics | null Allele: vps30-Δ | Other | Media: nitrogen starvation Details: autophagy assayed by delivery of cytoplasmic Pho8p to vacuole | Kihara A, et al. (2001) PMID:11157979 | |
| VPS30 | autophagy: absent | classical genetics | null Allele: vps30-Δ | S288C | Media: nitrogen starvation Details: class I mutant where vacuolar delivery of GFP-Atg8 is largely or almost fully abolished | Zhang Y, et al. (2020) PMID:31204567 | |
| VPS30 | autophagy: absent | classical genetics | null Allele: vps30-Δ | S288C | Media: potassium starvation Details: vacuolar processing of GFP-Atg8 is absent after 6 hours of potassium starvation, as opposed to wt cells where processing is observed at 3 hrs and peaks at 6 hrs | Rangarajan N, et al. (2020) PMID:32788210 | |
| VPS30 | autophagy: absent | classical genetics | null Allele: vps30-Δ | X2180-1A | Media: nitrogen starvation + 1 mM PMSF Details: autophagosomes not observed | Kametaka S, et al. (1998) PMID:9712845 | |
| VPS30 | autophagy: decreased | classical genetics | null Allele: vps30-Δ | S288C | 200 ng/ml sirolimus | Details: defective in bulk autophagy based on vacuolar delivery of GFP-Atg8 after rapamycin-induced starvation; also defective in ER-phagy based on vacuolar transport and degradation of Sec61-mCherry and Rtn1-GFP | Chen S, et al. (2020) PMID:32690699 |
| VPS30 | budding index: increased | homozygous diploid, large-scale survey | null Allele: vps30-Δ | S288C | Zettel MF, et al. (2003) PMID:12829295 | ||
| VPS30 | cell cycle progression in G1 phase: decreased duration | classical genetics | null Allele: vps30-Δ | S288C | Zettel MF, et al. (2003) PMID:12829295 | ||
| VPS30 | cell death: absent | classical genetics | null Allele: vps30-Δ | S288C | Treatment: human caspase-10 | Lisa-Santamaría P, et al. (2012) PMID:22782902 | |
| VPS30 | chemical compound accumulation: abnormal | systematic mutation set | null Allele: vps30-Δ | S288C | polyphosphate | Freimoser FM, et al. (2006) PMID:17107617 | |
| VPS30 | chemical compound accumulation: abnormal | systematic mutation set | null Allele: vps30-Δ | S288C | alpha-amino acid | Details: Significantly altered free amino acid profile (X^2- test adjusted p < 0.01), showing 2 simultaneous amino acid changes (Z-test, adjusted p < 0.01) | Mülleder M, et al. (2016) PMID:27693354 |
An interaction is defined as an experimentally observed physical or genetic interaction between two genes. There may be more than one row listed for the same interactor if the interaction between it and the given gene was observed in separate studies. All interactions listed in SGD are curated by BioGRID.
Increase the total number of rows showing on this page by using the pull-down located below the table, or use the page scroll at the table's top right to browse through the table's pages; use the arrows to the right of a column header to sort by that column; filter the table using the "Filter" box at the top of the table; click on the small "i" buttons located within a cell for an annotation to view further details about experiment type and any other genes involved in the interaction.
| Evidence ID | Analyze ID | Interactor | Interactor Systematic Name | Interactor | Interactor Systematic Name | Allele | Assay | Annotation | Action | Phenotype | SGA score | P-value | Source | Reference | Note |
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This diagram displays up to the top 30 positive interactions (green), negative interactions (orange), and phenotypes (blue) that are shared between the given allele (black) and other alleles (purple). The shared interactions or phenotypes can be visualized separately using the radio button at the bottom of the graph.
List of references used specifically to curate the information in Overview section.
Addgene Plasmids | DNASU Plasmids | PlasmID | Thermo Scientific | YGRC
dHITS | FitDB | HIPHOP Chemogenomics | HIP HOP Profiles | MetaboGeneCard | PROPHECY | SCMD | ScreenTroll | TheCellVision | Yeast Phenome
BioGRID | CYC2008 | DIP | GeneMANIA | IMP | InterologFinder | ModelArchive | TheCellMap | YeastRC Two-Hybrid