Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided.
3 entries for 3 phenotypesIncrease the total number of rows showing on this page using the pull-down located below the table, or use the page scroll at the table's top right to browse through the table's pages; use the arrows to the right of a column header to sort by that column; filter the table using the "Filter" box at the top of the table; click on the small "i" buttons located within a cell for an annotation to view further details.
| Gene | Phenotype | Experiment Type | Mutant Information | Strain Background | Chemical | Details | Reference |
|---|---|---|---|---|---|---|---|
| DPH1 | protein/peptide modification: absent Reporter: EF2 | classical genetics | null Allele: dph1-G238A G238A; conserved residue in the SAM-binding pocket mutated | S288C | Details: loss of diphthamide-dependent, ADP ribosylation of elongation factor 2 (EF2) relative to wt | Ütkür K, et al. (2023) PMID:38002337 | |
| DPH1 | resistance to chemicals: increased | classical genetics | null Allele: dph1-G238A G238A; conserved residue in the SAM-binding pocket mutated | S288C | 12.5 μg/ml sordarin | Details: increased resistance to sordarin, an antifungal inhibits EF2 in a fashion that depends on diphthamide; resistance is less than that observed in the null mutant and the dph1-D374A mutant | Ütkür K, et al. (2023) PMID:38002337 |
| DPH1 | toxin resistance: increased | classical genetics | null Allele: dph1-G238A G238A; conserved residue in the SAM-binding pocket mutated | S288C | Treatment: diphtheria toxin (DT), fragment A Details: increased resistance to overexpressed diphtheria toxin, fragment A; resistance is less than that observed with the null mutant | Ütkür K, et al. (2023) PMID:38002337 |
This diagram displays up to the top 30 positive interactions (green), negative interactions (orange), and phenotypes (blue) that are shared between the given allele (black) and other alleles (purple). The shared interactions or phenotypes can be visualized separately using the radio button at the bottom of the graph.
List of references used specifically to curate the information in Overview section.
Addgene Plasmids | DNASU Plasmids | PlasmID | Thermo Scientific | YGRC
dHITS | FitDB | HIPHOP Chemogenomics | HIP HOP Profiles | MetaboGeneCard | PROPHECY | SCMD | ScreenTroll | TheCellVision | Yeast Phenome
BioGRID | CYC2008 | DIP | GeneMANIA | IMP | InterologFinder | ModelArchive | TheCellMap | YeastRC Two-Hybrid