Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided.
3 entries for 2 phenotypesIncrease the total number of rows showing on this page using the pull-down located below the table, or use the page scroll at the table's top right to browse through the table's pages; use the arrows to the right of a column header to sort by that column; filter the table using the "Filter" box at the top of the table; click on the small "i" buttons located within a cell for an annotation to view further details.
| Gene | Phenotype | Experiment Type | Mutant Information | Strain Background | Chemical | Details | Reference |
|---|---|---|---|---|---|---|---|
| RGA1 | cellular morphology: abnormal | classical genetics | reduction of function Allele: rga1-Y650* Y650*; premature stop codon; removes the C-terminal GAP domain | Sigma1278b | Details: cells are hyperfilamentous and misshapen relative to the control, in a strain lacking mating-specific genes (ste4 null background), biasing activity towards the filamentous growth MAPK pathway | Pujari AN and Cullen PJ (2024) PMID:38560781 | |
| RGA1 | RNA accumulation: increased Reporter: FUS1-HIS3 mRNA | classical genetics | reduction of function Allele: rga1-Y650* Y650*; premature stop codon; removes the C-terminal GAP domain | Sigma1278b | Details: increased expression of a filamentous MAPK-pathway dependent growth reporter, FUS1-HIS3, in the absence of mating-specific genes (ste4 null background), biasing reporter activity towards the filamentous growth MAPK pathway; indicative of increased fMAPK activity; similar results with rga1-E663*, rga1-S679Fs, rga1-Q713*, rga1-E758*, rga1-C797*, rga1-E803*, rga1-L859* and rga1-G826S | Pujari AN and Cullen PJ (2024) PMID:38560781 | |
| RGA1 | RNA accumulation: increased Reporter: FUS1-lacZ mRNA | classical genetics | reduction of function Allele: rga1-Y650* Y650*; premature stop codon; removes the C-terminal GAP domain | Sigma1278b | Details: increased fMAPK activity based on increased expression of a fMAPK-pathway dependent reporter, FUS1-lacZ, in the absence of mating-specific genes (ste4 null background), biasing reporter activity towards the filamentous growth MAPK pathway; similar results with rga1-E663*, rga1-S679Fs, rga1-Q713*, rga1-E758*, rga1-C797*, rga1-E803*, rga1-L859* and rga1-G826S | Pujari AN and Cullen PJ (2024) PMID:38560781 |
This diagram displays up to the top 30 positive interactions (green), negative interactions (orange), and phenotypes (blue) that are shared between the given allele (black) and other alleles (purple). The shared interactions or phenotypes can be visualized separately using the radio button at the bottom of the graph.
List of references used specifically to curate the information in Overview section.
Addgene Plasmids | DNASU Plasmids | PlasmID | Thermo Scientific | YGRC
dHITS | FitDB | HIPHOP Chemogenomics | HIP HOP Profiles | MetaboGeneCard | PROPHECY | SCMD | ScreenTroll | TheCellVision
BioGRID | CYC2008 | DIP | GeneMANIA | IMP | InterologFinder | ModelArchive | TheCellMap | YeastRC Two-Hybrid