Complex: mRNA capping enzyme complex
Macromolecular complex annotations are imported from the Complex Portal. These annotations have been derived from physical molecular interaction evidence extracted from the literature and cross-referenced in the entry, or by curator inference from information on homologs in closely related species or by inference from scientific background.
- Curated by
- IntAct
- ComplexAc
- CPX-580
- Systematic Name
- CEG1:2xCET1
- SGD ID
- SGD:S000217864
- Aliases
- CET1-MCE1 complex ; Cet1-Ceg1 (triphosphatase-guanylyltransferase) complex ; mRNA guanylyltransferase complex
- Description
- Catalyzes the first step in mRNA cap formation. Consists of two subunits: an RNA 5'-triphosphatase (RTPase) and GTP:mRNA guanylyltransferase (GTase). The GTase subunit (CEG1) binds to the phosphorylated carboxyl-terminal domain of the largest subunit (CTD-P) of RNA polymerase II, coupling capping with transcription. CEG1 bound to the CTD-P is inactive unless allosterically activated by interaction with the RTPase subunit (CET1). CET1 removes the gamma-phosphate from the 5' end of the RNA substrate to leave a diphosphate end. CEG1 subsequently transfers the GMP moiety of GTP to the 5' end of RNA via an enzyme-GMP covalent reaction intermediate to form the structure GpppN1-.mRNA.
Complex Diagram
This diagram displays the protein subunits (blue) of the complex and how they interact with each other. Interactions with other relevant participants such as small molecules (purple), sub-complexes (yellow), and other subunits (red) are also shown. If a participant appears in the diagram with no lines connected to it, then its specific interactions with other participants are currently unknown.
PDB Structure
When available from the Protein Data Bank (PDB), the physical structure of the macromolecular complex is displayed. Click on the image or link for further information about the physical structure.
| 3KYH |
Subunits
This table lists all participants of the complex (proteins, small molecules, nucleic acids, etc.) and their respective stoichiometry. A brief description is also provided for each participant in the table.
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| Subunit | Description | Stoichiometry |
|---|
Gene Ontology
Gene Ontology (GO) terms that describe the function of a complex, the biological process in which it participates, or its cellular location. Click on a term for its definition and other objects annotated to that term.
- Molecular Function
-
- enables GTP binding (IPI)
- enables carbamoyl-phosphate synthase (glutamine-hydrolyzing) activity (IDA)
- enables mRNA guanylyltransferase activity (IDA)
- Biological Process
-
- involved in 7-methylguanosine mRNA capping (IDA)
Shared Annotations
This diagram displays Gene Ontology terms (green) and subunits (blue) that are shared between the given macromolecular complex (black) and other yeast complexes (yellow). The shared GO terms or subunits can be visualized separately using the radio button at the bottom of the graph. Note: No diagram is shown ("No shared annotations") if there are less than 2 shared annotations (either GO terms or subunits of other complexes) between this complex and any other complexes.
No shared annotations
References
List of references used specifically to curate the information on this page.
- Gu M and Lima CD (2005) Processing the message: structural insights into capping and decapping mRNA. Curr Opin Struct Biol 15(1):99-106 PMID: 15718140
Resources
IntAct | ModelArchive | PDB:3KYH ... Show all Show fewer