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2-(3-amino-3-carboxypropyl)histidine synthase complex Overview

GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code.

Summary
Essential for the first step of biosynthesis of diphthamide, a unique post-translationally modified histidine residue on EEF2 (P32324), a GTPase that is essential in the elongation step of translation. The complex catalyzes the addition of an aminocarboxypropyl (ACP) group to a specific histidine residue in EEF2 using S-adenosylmethionine as a substrate. A small iron-containing protein DPH3 (Q3E840) donates one Fe atom to convert the [3Fe-4S] cluster in DPH1-DPH2 to a functional [4Fe-4S] cluster during the radical-SAM enzyme catalytic cycle. the [4Fe-4S]2+ cluster in DPH1-DPH2 is reduced to [4Fe-4S]+ using dithionite as the reductant. The [4Fe-4S]+ cluster donates two electrons to SAM, cleaving it, forming an organometallic complex and releasing methylthioadenosine.The organometallic intermediate serves as a stabilized ACP radical and reacts with EEF2 to form an intermediate which is converted to the ACP-modified EEF2 product after loss of a hydrogen atom
GO Slim Terms

The yeast GO Slim terms are higher level terms that best represent the major S. cerevisiae biological processes, functions, and cellular components. The GO Slim terms listed here are the broader parent terms for the specific terms to which this gene product is annotated, and thus represent the more general processes, functions, and components in which it is involved.

transferase activity, transferase activity, transferring alkyl or aryl (other than methyl) groups, peptidyl-amino acid modification, cytoplasm

Biological Process

Manually curated GO annotations reflect our best understanding of the basic biological process for this protein complex. Manually curated annotations are assigned by curators based on published papers when available, or by curatorial statements if necessary. Curators periodically review all manually curated GO annotations for accuracy and completeness.

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Qualifier Gene Ontology Term Annotation Extension Evidence Source Assigned On Reference

Molecular Function

Manually curated GO annotations reflect our best understanding of the basic molecular function for this protein complex. Manually curated annotations are assigned by curators based on published papers when available, or by curatorial statements if necessary. Curators periodically review all manually curated GO annotations for accuracy and completeness.

Increase the total number of rows showing on this page using the pull-down located below the table, or use the page scroll at the table's top right to browse through the table's pages; use the arrows to the right of a column header to sort by that column; filter the table using the "Filter" box at the top of the table.

Qualifier Gene Ontology Term Annotation Extension Evidence Source Assigned On Reference

Cellular Component

Manually curated GO annotations reflect our best understanding of the basic cellular component for this protein complex. Manually curated annotations are assigned by curators based on published papers when available, or by curatorial statements if necessary. Curators periodically review all manually curated GO annotations for accuracy and completeness.

Increase the total number of rows showing on this page using the pull-down located below the table, or use the page scroll at the table's top right to browse through the table's pages; use the arrows to the right of a column header to sort by that column; filter the table using the "Filter" box at the top of the table.

Qualifier Gene Ontology Term Annotation Extension Evidence Source Assigned On Reference

Shared Annotations

This diagram displays Gene Ontology terms (green) that are shared between the given macromolecular complex (black) and other yeast complexes (yellow). Note: No diagram is shown ("No shared annotations") if there are less than 2 shared GO annotations between this complex and any other complexes.