AboutBlogDownloadExploreHelpGet Data
Gene Ontology Help

SESAME metabolic enzyme complex Overview

GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code.

Summary
Multi-enzyme complex which regulates the crosstalk between Histone H3 (HHt1/HHT2, P61830) methylation and phosphorylation by sensing glycolysis and glucose-derived serine metabolism. This leads to auto-regulation of the expression of the PYK1 pyruvate kinase. Complex activity is stimulated by glycolysis and serine metabolism to repress gene expression and confer cell resistance to oxidative stress. In the presence of sufficient glucose, PYK1 is activated, and SET1 (P38827) is recruited at the promoter of PYK1 by active RNA polymerase II. PYK1 phosphorylates Thr-12 of histone H3 using phosphoenolpyruvate as a substrate, while serine biosynthetic enzymes in SESAME generate serine from 3-phosphoglyceric acid to stimulate this phosphorylation. The COMPASS complex (CPX-1039) then recruits SESAME to the PYK1 coding region, allowing COMPASS to utilize SAM synthesized by SESAME to methylate H3 Lys-5. This interaction with COMPASS promotes SESAME recruitment, which phosphorylates H3 Thr-12 at the gene body and suppresses PYK1 transcription. The acetyl-coenzyme A synthetase ACS2 is required for the regulation of telomere silencing and cellular senescence. SESAME interacts with the SAS histone acetyltransferase complex (CPX-777) to promote histone H4 (HHF1/ HHF2) Lys-17 acetylation and the occupancy of the transcription factor , BDF1 (P35817), at subtelomeric regions. This interaction maintains telomere silencing by antagonizing the spreading of SIR2 (P06700) histone deacetylase along the telomeres, which is enhanced by acetate.
GO Slim Terms

The yeast GO Slim terms are higher level terms that best represent the major S. cerevisiae biological processes, functions, and cellular components. The GO Slim terms listed here are the broader parent terms for the specific terms to which this gene product is annotated, and thus represent the more general processes, functions, and components in which it is involved.

ligase activity, oxidoreductase activity, transferase activity, transferase activity, transferring alkyl or aryl (other than methyl) groups, chromatin organization, response to oxidative stress, response to stress

Biological Process

Manually curated GO annotations reflect our best understanding of the basic biological process for this protein complex. Manually curated annotations are assigned by curators based on published papers when available, or by curatorial statements if necessary. Curators periodically review all manually curated GO annotations for accuracy and completeness.

Increase the total number of rows showing on this page using the pull-down located below the table, or use the page scroll at the table's top right to browse through the table's pages; use the arrows to the right of a column header to sort by that column; filter the table using the "Filter" box at the top of the table.

Qualifier Gene Ontology Term Annotation Extension Evidence Source Assigned On Reference

Molecular Function

Manually curated GO annotations reflect our best understanding of the basic molecular function for this protein complex. Manually curated annotations are assigned by curators based on published papers when available, or by curatorial statements if necessary. Curators periodically review all manually curated GO annotations for accuracy and completeness.

Increase the total number of rows showing on this page using the pull-down located below the table, or use the page scroll at the table's top right to browse through the table's pages; use the arrows to the right of a column header to sort by that column; filter the table using the "Filter" box at the top of the table.

Qualifier Gene Ontology Term Annotation Extension Evidence Source Assigned On Reference

Shared Annotations

This diagram displays Gene Ontology terms (green) that are shared between the given macromolecular complex (black) and other yeast complexes (yellow). Note: No diagram is shown ("No shared annotations") if there are less than 2 shared GO annotations between this complex and any other complexes.

No shared annotations