BOI1 / YBL085W Overview
- Standard Name
- BOI1 1 2
- Systematic Name
- YBL085W
- SGD ID
- SGD:S000000181
- Aliases
- GIN7 10 , BOB1 2
- Feature Type
- ORF , Verified
- Description
- Protein involved in polar growth, functionally redundant with Boi2p; required for fusion of secretory vesicles with plasma membrane at sites of polarized growth; interacts with bud-emergence protein Bem1p; contains an SH3 (src homology 3) domain and a PH (pleckstrin homology) domain; relocalizes from bud neck to cytoplasm upon DNA replication stress; BOI1 has a paralog, BOI2, that arose from the whole genome duplication 3 4 5 6 7
- Name Description
- Bem1 (One) Interacting protein 3
- Paralog
- BOI2 5
- Comparative Info
-
Sequence
The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains.
- Summary
- BOI1 has a paralog, BOI2, that arose from the whole genome duplication
Analyze Sequence
S288C only
BLASTN | BLASTP | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species
BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi
S288C vs. other strains
Protein
Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.
- Summary
- Contains an SH3 (src homology 3) domain and a PH (pleckstrin homology) domain; relocalizes from bud neck to cytoplasm upon DNA replication stress
- Length (a.a.)
- 980
- Mol. Weight (Da)
- 109313.7
- Isoelectric Point
- 9.64
- Median Abundance (molecules/cell)
- 2526 +/- 1486
- Half-life (hr)
- 5.6
Alleles
Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results.
View all BOI1 alleles in SGD search
Gene Ontology
GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes.
- Summary
- Phospholipid-binding protein involved in actin filament organization, apical bud growth, and cytokinetic cell separation; localizes to plasma membrane, cytoplasm, bud neck, and shmoo tip
View computational annotations
Molecular Function
- Manually Curated
- enables phospholipid binding (IDA)
Biological Process
- Manually Curated
- involved in actin filament organization (IGI)
- involved in budding cell apical bud growth (IGI)
- involved in septum digestion after cytokinesis (IGI)
- involved in vesicle fusion to plasma membrane (IMP)
Cellular Component
- Manually Curated
- is active in cellular bud (IDA)
- located in cellular bud neck (HDA, IDA)
- located in cytoplasm (HDA)
- located in mating projection tip (HDA)
- located in plasma membrane (HDA)
- located in site of polarized growth (IDA)
Phenotype
Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided. Click "Phenotype Details" to view all phenotype annotations and evidence for this locus as well as phenotypes it shares with other genes.
- Summary
- Non-essential gene; null mutant displays decreased competitive fitness in minimal medium; homozygous diploid null mutant has decreased oxidative stress resistance; null mutant displays increased resistance to treatment with bleomycin, camptothecin, cycloheximide and miconazole; overexpression results in a growth defect, increased cell size, a round morphology and an abnormal budding index with an increased quantity of unbudded cells; heterozygous diploid null is haploinsufficient
Classical Genetics
- null
- overexpression
Large-scale Survey
Interaction
Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. An interaction annotation is composed of the interaction type, name of the interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a reference, as well as other experimental details. Click "Interaction Details" to view all interaction annotations and evidence for this locus, including an interaction visualization.
- Summary
- The boi1 null mutant is viable; the null mutant of paralog boi2 is viable; the boi1 boi2 double mutant displays a growth defect.
277 total interactions for 184 unique genes
Physical Interactions
- Affinity Capture-MS: 46
- Affinity Capture-RNA: 8
- Affinity Capture-Western: 3
- Biochemical Activity: 3
- Co-localization: 3
- PCA: 40
- Protein-peptide: 2
- Proximity Label-MS: 1
- Reconstituted Complex: 11
- Two-hybrid: 63
Genetic Interactions
- Dosage Rescue: 12
- Negative Genetic: 59
- Phenotypic Enhancement: 5
- Phenotypic Suppression: 2
- Positive Genetic: 9
- Synthetic Growth Defect: 6
- Synthetic Lethality: 4
Regulation
The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the given locus, based on experimental evidence. This evidence includes data generated through high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO enrichment among regulation Targets, and a regulator/target diagram for the locus.
- Regulators
- 10
- Targets
- 0
Expression
Expression data are derived from records contained in the Gene Expression Omnibus (GEO), and are first log2 transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result there may be a greater number of conditions than datasets represented in a single clickable histogram bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from those that are up-regulated (red). Click "Expression Details" to view all expression annotations and details for this locus, including a visualization of genes that share a similar expression pattern.
Summary Paragraph
A summary of the locus, written by SGD Biocurators following a thorough review of the literature. Links to gene names and curated GO terms are included within the Summary Paragraphs.
Last Updated: 1999-04-29
Literature
All manually curated literature for the specified gene, organized into topics according to their relevance to the gene (Primary Literature, Additional Literature, or Review). Click "Literature Details" to view all literature information for this locus, including shared literature between genes.