COX15 / YER141W Overview
- Standard Name
- COX15 1
- Systematic Name
- YER141W
- SGD ID
- SGD:S000000943
- Feature Type
- ORF , Verified
- Description
- Heme a synthase; required for the hydroxylation of heme O to form heme A, an essential prosthetic group for cytochrome c oxidase; oligomerization is required for function 2 3 4
- Name Description
- Cytochrome c OXidase 1
- Comparative Info
-
Sequence
The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains.
Analyze Sequence
S288C only
BLASTN | BLASTP | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species
BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi
S288C vs. other strains
Protein
Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.
- Summary
- Cox15p is 486 amino acids long, extremely long-lived (3x average), low in abundance; acetylated on K281, phosphorylated on S476, succinylated on 6 residues
- Length (a.a.)
- 486
- Mol. Weight (Da)
- 54690.2
- Isoelectric Point
- 10.83
- Median Abundance (molecules/cell)
- 6031 +/- 2667
- Half-life (hr)
- 32.5
Alleles
Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results.
View all COX15 alleles in SGD search
Gene Ontology
GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes.
- Summary
- COX15/YER141W is a mitochondrial inner membrane oxidoreductase involved in biosynthesis of heme A
View computational annotations
Molecular Function
- Manually Curated
Biological Process
- Manually Curated
- involved in heme A biosynthetic process (IMP, IGI)
Cellular Component
- Manually Curated
- located in mitochondrial inner membrane (IDA)
- located in mitochondrion (HDA)
Phenotype
Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided. Click "Phenotype Details" to view all phenotype annotations and evidence for this locus as well as phenotypes it shares with other genes.
- Summary
- COX15/YER141W is a non-essential gene; null mutant is incapable of respiratory growth on nonfermentable carbon sources and grows slowly on fermentable carbon sources (petite phenotype); null mutant also shows increased frequency of nonfunctional mitochondrial genome (rho- mutations); under competitive growth conditions null mutant is sensitive to oxidative stress caused by hydrogen peroxide; in systematic studies mutants exhibit reduced biofilm formation, decreased competitive fitness and resistance to miconazole
Classical Genetics
- null
- biofilm formation: decreased
- chemical compound accumulation: decreased
- chemical compound excretion: increased
- competitive fitness: decreased
- desiccation resistance: decreased
- innate thermotolerance: decreased
- oxidative stress resistance: decreased
- resistance to chemicals: decreased
- resistance to chemicals: increased
- respiratory growth: absent
- respiratory growth: decreased rate
- stress resistance: increased
- utilization of nitrogen source: decreased rate
- vacuolar morphology: abnormal
- vegetative growth: decreased rate
- viable
- unspecified
Large-scale Survey
Disease
Disease Annotations consist of three mandatory components: a gene product, a term from the Disease Ontology (DO) controlled vocabulary and an evidence code. SGD provides manually curated DO Annotations derived from the literature. Click "Disease Details" to view all Disease information and evidence for this locus as well as diseases it shares with other genes.
- Summary
- Yeast COX15 is homologous to human COX15 and has been used to study mutations found in patients with oocyte development defects and female infertility
Manually Curated
Interaction
Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. An interaction annotation is composed of the interaction type, name of the interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a reference, as well as other experimental details. Click "Interaction Details" to view all interaction annotations and evidence for this locus, including an interaction visualization.
- Summary
- Cox15p interacts physically with proteins involved in mitochondrion organization; COX15 interacts genetically with genes involved in rRNA processing and transcription
88 total interactions for 82 unique genes
Physical Interactions
- Affinity Capture-MS: 14
- Affinity Capture-RNA: 7
- Affinity Capture-Western: 13
- Co-localization: 1
- Co-purification: 1
- Proximity Label-MS: 7
- Reconstituted Complex: 1
- Two-hybrid: 1
Genetic Interactions
- Dosage Growth Defect: 2
- Negative Genetic: 37
- Phenotypic Enhancement: 1
- Phenotypic Suppression: 1
- Positive Genetic: 2
Regulation
The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the given locus, based on experimental evidence. This evidence includes data generated through high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO enrichment among regulation Targets, and a regulator/target diagram for the locus.
- Regulators
- 5
- Targets
- 0
Expression
Expression data are derived from records contained in the Gene Expression Omnibus (GEO), and are first log2 transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result there may be a greater number of conditions than datasets represented in a single clickable histogram bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from those that are up-regulated (red). Click "Expression Details" to view all expression annotations and details for this locus, including a visualization of genes that share a similar expression pattern.
Summary Paragraph
A summary of the locus, written by SGD Biocurators following a thorough review of the literature. Links to gene names and curated GO terms are included within the Summary Paragraphs.
Last Updated: 2006-07-20
Literature
All manually curated literature for the specified gene, organized into topics according to their relevance to the gene (Primary Literature, Additional Literature, or Review). Click "Literature Details" to view all literature information for this locus, including shared literature between genes.