SUA7 / YPR086W Overview
- Standard Name
- SUA7 1
- Systematic Name
- YPR086W
- SGD ID
- SGD:S000006290
- Aliases
- TFIIB 2 , SOH4 11 12
- Feature Type
- ORF , Verified
- Description
- Transcription factor TFIIB; a general transcription factor required for transcription initiation and start site selection by RNA polymerase II 2 3 4 5 6
- Name Description
- Suppressor of Upstream AUG 1
- Comparative Info
-
Sequence
The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains.
Analyze Sequence
S288C only
BLASTN | BLASTP | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species
BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi
S288C vs. other strains
Protein
Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.
- Length (a.a.)
- 345
- Mol. Weight (Da)
- 38208.9
- Isoelectric Point
- 8.81
- Median Abundance (molecules/cell)
- 4262 +/- 1425
- Half-life (hr)
- 7.1
Alleles
Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results.
View all SUA7 alleles in SGD search
Gene Ontology
GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes.
- Summary
- General transcription factor with roles in RNA polymerase II preinitiation complex assembly, transcription start site selection and formation of transcription open complex at Polymerase II promoters; localized to the nucleus
View computational annotations
Molecular Function
- Manually Curated
- enables RNA polymerase II complex binding (IDA, IPI)
- enables RNA polymerase II complex recruiting activity (IMP, IDA)
- enables TBP-class protein binding (IMP, IDA, IPI)
- enables zinc ion binding (RCA)
Biological Process
- Manually Curated
- involved in positive regulation of transcription regulatory region DNA binding (IDA)
- involved in RNA polymerase II preinitiation complex assembly (IMP, IDA)
- involved in transcription open complex formation at RNA polymerase II promoter (IMP)
- involved in transcriptional start site selection at RNA polymerase II promoter (IMP)
Cellular Component
- Manually Curated
- located in nucleus (IDA)
- part of transcription preinitiation complex (IDA)
Phenotype
Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided. Click "Phenotype Details" to view all phenotype annotations and evidence for this locus as well as phenotypes it shares with other genes.
- Summary
- Essential gene; reduction-of-function mutations lead to increased cold and heat sensitivity, and slow growth under standard conditions; heterozygous diploids show haploinsufficiency
Classical Genetics
- null
- reduction of function
- null
- reduction of function
- conditional
Large-scale Survey
Interaction
Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. An interaction annotation is composed of the interaction type, name of the interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a reference, as well as other experimental details. Click "Interaction Details" to view all interaction annotations and evidence for this locus, including an interaction visualization.
3480 total interactions for 3097 unique genes
Physical Interactions
- Affinity Capture-MS: 2990
- Affinity Capture-RNA: 3
- Affinity Capture-Western: 27
- Biochemical Activity: 1
- Co-crystal Structure: 5
- Co-localization: 6
- Co-purification: 7
- Far Western: 1
- PCA: 5
- Reconstituted Complex: 23
- Two-hybrid: 5
Genetic Interactions
- Dosage Rescue: 11
- Negative Genetic: 291
- Phenotypic Enhancement: 3
- Phenotypic Suppression: 1
- Positive Genetic: 70
- Synthetic Growth Defect: 3
- Synthetic Lethality: 12
- Synthetic Rescue: 16
Regulation
The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the given locus, based on experimental evidence. This evidence includes data generated through high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO enrichment among regulation Targets, and a regulator/target diagram for the locus.
- Summary
- SUA7 encodes the general transcription factor TFIIB, which is required for the initiation of promoter-dependent transcription by RNA polymerase II (RNAPII). TFIIB and other general transcription factors assemble with RNAPII and promoter DNA in a large complex at every round of transcription initiation. TFIIB and the TATA-binding protein (TBP) subunit of TFIID are primarily responsible for promoter recognition. TFIIB has been implicated in both start site selection and in stabilization of the initial transcript. The N-terminus of TFIIB includes a zinc ribbon domain which contacts the dock domain of RNAPII, and a loop termed the B finger that reaches into the active center of the RNAPII and interacts with both DNA and RNA. The C-terminus of TFIIB, which contains a series of cyclin repeats that make up the C-terminal core, locates above the RNAPII active center cleft. The central linker region of TFIIB snakes down from above the cleft toward the active center, past loops of Rpo21p and Rpb2p, and the Rpo21p clamp, contributing to stabilization of those structures. DNA follows a path above the RNAPII central cleft leading to the RNAPII active center. TFIIB and RNAPII form a template-strand tunnel which promotes transient and progressive promoter melting, contributing to the closed-to-open promoter transition, and maintaining the transcription bubble until the DNA-RNA hybrid is long enough to persist on its own. TFIIB may also serve as a scaffold for binding other transcription factors.
Expression
Expression data are derived from records contained in the Gene Expression Omnibus (GEO), and are first log2 transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result there may be a greater number of conditions than datasets represented in a single clickable histogram bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from those that are up-regulated (red). Click "Expression Details" to view all expression annotations and details for this locus, including a visualization of genes that share a similar expression pattern.
Summary Paragraph
A summary of the locus, written by SGD Biocurators following a thorough review of the literature. Links to gene names and curated GO terms are included within the Summary Paragraphs.
Last Updated: 2000-01-04
Literature
All manually curated literature for the specified gene, organized into topics according to their relevance to the gene (Primary Literature, Additional Literature, or Review). Click "Literature Details" to view all literature information for this locus, including shared literature between genes.