HSE1 / YHL002W Overview
- Standard Name
- HSE1 1
- Systematic Name
- YHL002W
- SGD ID
- SGD:S000000994
- Feature Type
- ORF , Verified
- Description
- Subunit of the endosomal Vps27p-Hse1p complex; complex is required for sorting of ubiquitinated membrane proteins into intralumenal vesicles prior to vacuolar degradation, as well as for recycling of Golgi proteins and formation of lumenal membranes 1 2
- Name Description
- Has Symptoms of class E mutants; resembles Hbp, Stam and East 1
- Comparative Info
-
Sequence
The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains.
- Summary
- HSE1/YHL002W is located on the left arm of chromosome VIII very near the centromere between LAG1 and RPL14B; coding sequence is 1359 nucleotides long with 10 SNPs, 5 of which cause amino acid polymorphisms
Analyze Sequence
S288C only
BLASTN | BLASTP | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species
BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi
S288C vs. other strains
Protein
Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.
- Summary
- Hse1p is 452 amino acids long, short-lived, low in abundance; contains 4 SH3 domains; ubiquitinylated on 4 lysines, phosphorylated on 3 serines
- Length (a.a.)
- 452
- Mol. Weight (Da)
- 51155.2
- Isoelectric Point
- 6.37
- Median Abundance (molecules/cell)
- 2756 +/- 994
- Half-life (hr)
- 4.3
Alleles
Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results.
View all HSE1 alleles in SGD search
Gene Ontology
GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes.
- Summary
- Ubiquitin-binding subunit of ESCRT-0 complex; involved in ATP export, vacuolar protein targeting, lysosomal microautophagy, and late endosome-to-vacuole transport; localizes to endosome and vacuolar membrane
View computational annotations
Molecular Function
- Manually Curated
- enables ubiquitin binding (IDA)
Biological Process
- Manually Curated
- involved in ATP export (IMP)
- involved in late endosome to vacuole transport (IMP)
- involved in microautophagy (IMP)
- involved in protein targeting to vacuole (IMP, IPI)
Cellular Component
- Manually Curated
- located in endosome (IDA)
- part of ESCRT-0 complex (IPI)
- located in vacuolar membrane (IDA)
Complex
Macromolecular complex annotations are imported from the Complex Portal. These annotations have been derived from physical molecular interaction evidence extracted from the literature and cross-referenced in the entry, or by curator inference from information on homologs in closely related species or by inference from scientific background.
Phenotype
Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided. Click "Phenotype Details" to view all phenotype annotations and evidence for this locus as well as phenotypes it shares with other genes.
- Summary
- HSE1/YHL002W is a non-essential gene; null mutants are viable and exhibit a variety of phenotypic changes, including increased biofilm formation and replicative lifespan, but decreased chitin deposition, competitive fitness, endocytosis, and oxidative stress resistance. Null mutants also display abnormal vacuolar morphology. Reduction of function mutants show decreased vacuolar transport. Overexpression of HSE1/YHL002W results in abnormal budding and cellular morphology, as well as decreased vegetative growth.
Classical Genetics
- null
- biofilm formation: increased
- chemical compound accumulation: abnormal
- chemical compound accumulation: increased
- chitin deposition: decreased
- competitive fitness: decreased
- endocytosis: decreased
- haploproficient
- oxidative stress resistance: decreased
- replicative lifespan: increased
- resistance to chemicals: decreased
- resistance to chemicals: increased
- stress resistance: decreased
- vacuolar morphology: abnormal
- viable
- overexpression
Large-scale Survey
Interaction
Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. An interaction annotation is composed of the interaction type, name of the interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a reference, as well as other experimental details. Click "Interaction Details" to view all interaction annotations and evidence for this locus, including an interaction visualization.
- Summary
- Hse1p interacts physically with proteins involved in transcription; HSE1 interacts genetically with genes involved in transcription
452 total interactions for 350 unique genes
Physical Interactions
- Affinity Capture-MS: 16
- Affinity Capture-RNA: 2
- Affinity Capture-Western: 13
- Co-crystal Structure: 2
- Co-localization: 1
- Co-purification: 1
- PCA: 8
- Protein-peptide: 1
- Proximity Label-MS: 1
- Reconstituted Complex: 8
- Two-hybrid: 53
Genetic Interactions
- Dosage Rescue: 1
- Negative Genetic: 278
- Phenotypic Enhancement: 1
- Positive Genetic: 51
- Synthetic Growth Defect: 9
- Synthetic Lethality: 2
- Synthetic Rescue: 4
Regulation
The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the given locus, based on experimental evidence. This evidence includes data generated through high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO enrichment among regulation Targets, and a regulator/target diagram for the locus.
- Regulators
- 4
- Targets
- 0
Expression
Expression data are derived from records contained in the Gene Expression Omnibus (GEO), and are first log2 transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result there may be a greater number of conditions than datasets represented in a single clickable histogram bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from those that are up-regulated (red). Click "Expression Details" to view all expression annotations and details for this locus, including a visualization of genes that share a similar expression pattern.
Summary Paragraph
A summary of the locus, written by SGD Biocurators following a thorough review of the literature. Links to gene names and curated GO terms are included within the Summary Paragraphs.
Last Updated: 2025-01-27
Literature
All manually curated literature for the specified gene, organized into topics according to their relevance to the gene (Primary Literature, Additional Literature, or Review). Click "Literature Details" to view all literature information for this locus, including shared literature between genes.