HSE1 / YHL002W Overview


Standard Name
HSE1 1
Systematic Name
YHL002W
SGD ID
SGD:S000000994
Feature Type
ORF , Verified
Description
Subunit of the endosomal Vps27p-Hse1p complex; complex is required for sorting of ubiquitinated membrane proteins into intralumenal vesicles prior to vacuolar degradation, as well as for recycling of Golgi proteins and formation of lumenal membranes 1 2
Name Description
Has Symptoms of class E mutants; resembles Hbp, Stam and East 1
Comparative Info
Sequence Details

Sequence

The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains.


Summary
HSE1/YHL002W is located on the left arm of chromosome VIII very near the centromere between LAG1 and RPL14B; coding sequence is 1359 nucleotides long with 10 SNPs, 5 of which cause amino acid polymorphisms
Protein Details

Protein

Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.


Summary
Hse1p is 452 amino acids long, short-lived, low in abundance; contains 4 SH3 domains; ubiquitinylated on 4 lysines, phosphorylated on 3 serines
Length (a.a.)
452
Mol. Weight (Da)
51155.2
Isoelectric Point
6.37
Median Abundance (molecules/cell)
2756 +/- 994
Half-life (hr)
4.3

Alleles

Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results.


View all HSE1 alleles in SGD search

Gene Ontology Details

Gene Ontology

GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes.


Summary
Ubiquitin-binding subunit of ESCRT-0 complex; involved in ATP export, vacuolar protein targeting, lysosomal microautophagy, and late endosome-to-vacuole transport; localizes to endosome and vacuolar membrane

View computational annotations

Molecular Function

Manually Curated

Biological Process

Manually Curated

Cellular Component

Manually Curated

Complex

Macromolecular complex annotations are imported from the Complex Portal. These annotations have been derived from physical molecular interaction evidence extracted from the literature and cross-referenced in the entry, or by curator inference from information on homologs in closely related species or by inference from scientific background.


Phenotype Details

Phenotype

Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided. Click "Phenotype Details" to view all phenotype annotations and evidence for this locus as well as phenotypes it shares with other genes.


Summary
HSE1/YHL002W is a non-essential gene; null mutants are viable and exhibit a variety of phenotypic changes, including increased biofilm formation and replicative lifespan, but decreased chitin deposition, competitive fitness, endocytosis, and oxidative stress resistance. Null mutants also display abnormal vacuolar morphology. Reduction of function mutants show decreased vacuolar transport. Overexpression of HSE1/YHL002W results in abnormal budding and cellular morphology, as well as decreased vegetative growth.
Interaction Details

Interaction

Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. An interaction annotation is composed of the interaction type, name of the interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a reference, as well as other experimental details. Click "Interaction Details" to view all interaction annotations and evidence for this locus, including an interaction visualization.


Summary
Hse1p interacts physically with proteins involved in transcription; HSE1 interacts genetically with genes involved in transcription

452 total interactions for 350 unique genes

Physical Interactions

  • Affinity Capture-MS: 16
  • Affinity Capture-RNA: 2
  • Affinity Capture-Western: 13
  • Co-crystal Structure: 2
  • Co-localization: 1
  • Co-purification: 1
  • PCA: 8
  • Protein-peptide: 1
  • Proximity Label-MS: 1
  • Reconstituted Complex: 8
  • Two-hybrid: 53

Genetic Interactions

  • Dosage Rescue: 1
  • Negative Genetic: 278
  • Phenotypic Enhancement: 1
  • Positive Genetic: 51
  • Synthetic Growth Defect: 9
  • Synthetic Lethality: 2
  • Synthetic Rescue: 4
Regulation Details

Regulation

The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the given locus, based on experimental evidence. This evidence includes data generated through high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO enrichment among regulation Targets, and a regulator/target diagram for the locus.


Summary
HSE1 promoter is bound by Rfx1p and Yap6p in response to heat
Regulators
4
Targets
0
Expression Details

Expression

Expression data are derived from records contained in the Gene Expression Omnibus (GEO), and are first log2 transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result there may be a greater number of conditions than datasets represented in a single clickable histogram bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from those that are up-regulated (red). Click "Expression Details" to view all expression annotations and details for this locus, including a visualization of genes that share a similar expression pattern.


Summary Paragraph

A summary of the locus, written by SGD Biocurators following a thorough review of the literature. Links to gene names and curated GO terms are included within the Summary Paragraphs.


Last Updated: 2025-01-27

Literature Details

Literature

All manually curated literature for the specified gene, organized into topics according to their relevance to the gene (Primary Literature, Additional Literature, or Review). Click "Literature Details" to view all literature information for this locus, including shared literature between genes.


Primary
31
Additional
30
Reviews
31

Resources