KIN28 / YDL108W Overview
- Standard Name
- KIN28 1
- Systematic Name
- YDL108W
- SGD ID
- SGD:S000002266
- Feature Type
- ORF , Verified
- Description
- Ser/Thr protein kinase and subunit of TFIIK, a TFIIH subassembly; phosphorylates the C-terminal domain (CTD) of RNAPII (Ser5 and Ser7); facilitates recruitment of mRNA 5'-capping and polyadenylation factors to the RNAPII holoenzyme complex; minor role in RNAPII transcription; role in RNAPI transcription; regulates Atg1p kinase complex assembly during nitrogen starvation induced autophagy; associates with cyclin Ccl1p; relocalizes to the cytosol in response to hypoxia; ortholog of human CDK7 2 3 4 5 6 7 8 9
- Name Description
- protein KINase
- Comparative Info
-
Sequence
The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains.
Analyze Sequence
S288C only
BLASTN | BLASTP | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species
BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi
S288C vs. other strains
Protein
Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.
- Summary
- Kin28p is 306 amino acids long, extremely long-lived (4x average), low in abundance; phosphorylated on 3 residues; relocalizes to the cytosol in response to hypoxia
- Length (a.a.)
- 306
- Mol. Weight (Da)
- 35245.3
- Isoelectric Point
- 6.88
- Median Abundance (molecules/cell)
- 2151 +/- 704
- Half-life (hr)
- 42.7
Alleles
Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results.
View all KIN28 alleles in SGD search
Gene Ontology
GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes.
- Summary
- RNA polymerase II CTD heptapeptide repeat kinase involved in regulation of transcription and mRNA capping; part of transcription factor complexes TFIIH and TFIIK; shuttles between nucleus and cytoplasm
View computational annotations
Molecular Function
- Manually Curated
- enables protein kinase activity (HDA)
- enables RNA polymerase II CTD heptapeptide repeat kinase activity (IMP, IDA)
Biological Process
- Manually Curated
- involved in 7-methylguanosine mRNA capping (IMP)
- involved in positive regulation of autophagy (IMP)
- involved in positive regulation of transcription by RNA polymerase II (IDA)
- involved in positive regulation of transcription elongation by RNA polymerase II (IMP)
- involved in transcription by RNA polymerase I (IMP)
- involved in transcription by RNA polymerase II (IMP, IDA)
Cellular Component
- Manually Curated
- located in cytosol (IDA)
- located in nucleus (IDA)
- part of transcription factor TFIIH holo complex (IDA)
- part of transcription factor TFIIK complex (IDA)
Complex
Macromolecular complex annotations are imported from the Complex Portal. These annotations have been derived from physical molecular interaction evidence extracted from the literature and cross-referenced in the entry, or by curator inference from information on homologs in closely related species or by inference from scientific background.
Phenotype
Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided. Click "Phenotype Details" to view all phenotype annotations and evidence for this locus as well as phenotypes it shares with other genes.
Classical Genetics
- null
- conditional
- repressible
- null
- reduction of function
- conditional
Large-scale Survey
Disease
Disease Annotations consist of three mandatory components: a gene product, a term from the Disease Ontology (DO) controlled vocabulary and an evidence code. SGD provides manually curated DO Annotations derived from the literature. Click "Disease Details" to view all Disease information and evidence for this locus as well as diseases it shares with other genes.
- Summary
- Yeast KIN28 is homologous to human ACVR1C, AKT2, BMPR1B, and GRK4, and has been used to study primary congenital glaucoma, type 2 diabetes mellitus, hypoglycemia, brachydactyly type A2, breast cancer, and essential hypertension
High-Throughput
- is_implicated_in brachydactyly type A2 (IGI, ISS)
- is_implicated_in breast cancer (IGI, ISS)
- is_implicated_in essential hypertension (IGI, ISS)
- is_implicated_in hypoglycemia (IGI, ISS)
- is_implicated_in primary congenital glaucoma (ISS, IGI)
- is_implicated_in type 2 diabetes mellitus (IGI, ISS)
Interaction
Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. An interaction annotation is composed of the interaction type, name of the interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a reference, as well as other experimental details. Click "Interaction Details" to view all interaction annotations and evidence for this locus, including an interaction visualization.
713 total interactions for 559 unique genes
Physical Interactions
- Affinity Capture-MS: 25
- Affinity Capture-RNA: 3
- Affinity Capture-Western: 13
- Biochemical Activity: 22
- Co-crystal Structure: 1
- Co-localization: 3
- Co-purification: 10
- Far Western: 2
- Reconstituted Complex: 27
- Two-hybrid: 7
Genetic Interactions
- Dosage Lethality: 2
- Dosage Rescue: 8
- Negative Genetic: 432
- Phenotypic Suppression: 2
- Positive Genetic: 131
- Synthetic Growth Defect: 7
- Synthetic Lethality: 15
- Synthetic Rescue: 3
Regulation
The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the given locus, based on experimental evidence. This evidence includes data generated through high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO enrichment among regulation Targets, and a regulator/target diagram for the locus.
Expression
Expression data are derived from records contained in the Gene Expression Omnibus (GEO), and are first log2 transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result there may be a greater number of conditions than datasets represented in a single clickable histogram bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from those that are up-regulated (red). Click "Expression Details" to view all expression annotations and details for this locus, including a visualization of genes that share a similar expression pattern.
Summary Paragraph
A summary of the locus, written by SGD Biocurators following a thorough review of the literature. Links to gene names and curated GO terms are included within the Summary Paragraphs.
Last Updated: 2024-11-14
Literature
All manually curated literature for the specified gene, organized into topics according to their relevance to the gene (Primary Literature, Additional Literature, or Review). Click "Literature Details" to view all literature information for this locus, including shared literature between genes.