PMP3 / YDR276C Overview
- Standard Name
- PMP3 1
- Systematic Name
- YDR276C
- SGD ID
- SGD:S000002684
- Aliases
- SNA1 4
- Feature Type
- ORF , Verified
- Description
- Small plasma membrane protein; confers resistance to amphotericin B and is a potential target of this common antifungal drug; related to a family of plant polypeptides that are overexpressed under high salt concentration or low temperature; not essential for viability; deletion causes hyperpolarization of the plasma membrane potential 1 2
- Name Description
- Plasma Membrane Proteolipid 3
- Comparative Info
-
Sequence
The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains.
Analyze Sequence
S288C only
BLASTN | BLASTP | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species
BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi
S288C vs. other strains
Protein
Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.
- Summary
- Pmp3p is 55 amino acids long, high in abundance; shares domains with proteins involved in transport
- Length (a.a.)
- 55
- Mol. Weight (Da)
- 6136.5
- Isoelectric Point
- 4.02
- Median Abundance (molecules/cell)
- 23654
Alleles
Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results.
View all PMP3 alleles in SGD search
Gene Ontology
GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes.
- Summary
- Protein that binds to phosphatidylinositol-3-phosphate, phosphatidylinositol-3,5-bisphosphate, phosphatidic acid, and sphingolipids; involved in cation transport and regulation of membrane potential; localized to the plasma membrane
View computational annotations
Molecular Function
- Manually Curated
- enables phosphatidic acid binding (IDA)
- enables phosphatidylinositol-3,5-bisphosphate binding (IDA)
- enables phosphatidylinositol-3-phosphate binding (IDA)
- enables sphingolipid binding (IDA)
Biological Process
- Manually Curated
- involved in monoatomic cation transport (IMP)
- involved in regulation of membrane potential (IMP)
Cellular Component
- Manually Curated
- located in cell periphery (HDA)
- located in plasma membrane (IDA)
Phenotype
Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided. Click "Phenotype Details" to view all phenotype annotations and evidence for this locus as well as phenotypes it shares with other genes.
- Summary
- Non-essential gene; null has elevation of glutathione excretion, defects in endocytosis and actin polarization, decreased lifespan, is sensitive to salt, acid, metals, Congo Red, antifungals, caffeine, heat, cycloheximide; overexpression slows growth, increases resistance to amphotericin B and to myriocin
Classical Genetics
- null
- actin cytoskeleton morphology: abnormal
- chemical compound accumulation: decreased
- chemical compound accumulation: increased
- endocytosis: decreased rate
- ionic stress resistance: decreased
- protein/peptide accumulation: abnormal
- protein/peptide accumulation: decreased
- protein/peptide accumulation: increased
- protein/peptide modification: decreased
- resistance to chemicals: decreased
- resistance to chemicals: increased
- RNA accumulation: increased
- overexpression
- null
- auxotrophy
- chemical compound accumulation: abnormal
- chemical compound excretion: increased
- chitin deposition: normal
- chronological lifespan: decreased
- colony appearance: abnormal
- competitive fitness: decreased
- desiccation resistance: decreased
- endocytosis: decreased
- haploproficient
- heat sensitivity: increased
- hyperosmotic stress resistance: decreased
- protein secretion: increased
- resistance to chemicals: decreased
- resistance to chemicals: increased
- respiratory growth: decreased rate
- stress resistance: decreased
- toxin resistance: increased
- viable
- overexpression
- unspecified
- conditional
Large-scale Survey
Interaction
Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. An interaction annotation is composed of the interaction type, name of the interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a reference, as well as other experimental details. Click "Interaction Details" to view all interaction annotations and evidence for this locus, including an interaction visualization.
- Summary
- Pmp3p interacts physically with proteins involved in translation regulation; PMP3 interacts genetically with genes involved in transcription
749 total interactions for 520 unique genes
Physical Interactions
- Affinity Capture-RNA: 8
- Affinity Capture-Western: 2
- Co-purification: 1
- PCA: 19
- Two-hybrid: 2
Genetic Interactions
- Dosage Lethality: 1
- Dosage Rescue: 4
- Negative Genetic: 560
- Phenotypic Suppression: 3
- Positive Genetic: 82
- Synthetic Growth Defect: 45
- Synthetic Lethality: 9
- Synthetic Rescue: 13
Regulation
The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the given locus, based on experimental evidence. This evidence includes data generated through high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO enrichment among regulation Targets, and a regulator/target diagram for the locus.
- Regulators
- 20
- Targets
- 0
Expression
Expression data are derived from records contained in the Gene Expression Omnibus (GEO), and are first log2 transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result there may be a greater number of conditions than datasets represented in a single clickable histogram bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from those that are up-regulated (red). Click "Expression Details" to view all expression annotations and details for this locus, including a visualization of genes that share a similar expression pattern.
Literature
All manually curated literature for the specified gene, organized into topics according to their relevance to the gene (Primary Literature, Additional Literature, or Review). Click "Literature Details" to view all literature information for this locus, including shared literature between genes.