PTC1 / YDL006W Overview
- Standard Name
- PTC1 1
- Systematic Name
- YDL006W
- SGD ID
- SGD:S000002164
- Aliases
- CWH47 10 , KCS2 11 , TPD1 2
- Feature Type
- ORF , Verified
- Description
- Type 2C protein phosphatase (PP2C); dephosphorylates Hog1p, inactivating the osmosensing MAPK cascade; involved in Fus3p activation during pheromone response; deletion affects precursor tRNA splicing, mitochondrial inheritance, and sporulation; involved in myosin-V dependent inheritance of mitochondria, peroxisomes, vacuoles and secretory vesicles; localizes to the nucleus, cytoplasm and peroxisomes 2 3 4 5 6 7 8 9
- Name Description
- Phosphatase type Two C 1
- Comparative Info
-
Sequence
The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains.
Analyze Sequence
S288C only
BLASTN | BLASTP | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species
BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi
S288C vs. other strains
Protein
Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.
- Summary
- Ptc1p is 281 amino acids long, of average half-life, very low in abundance; ubiquitinylated on K188, phosphorylated on 6 residues
- Length (a.a.)
- 281
- Mol. Weight (Da)
- 31534.8
- Isoelectric Point
- 4.64
- Median Abundance (molecules/cell)
- 1520 +/- 690
- Half-life (hr)
- 10.4
Alleles
Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results.
View all PTC1 alleles in SGD search
Gene Ontology
GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes.
- Summary
- Protein serine/threonine phosphatase involved in the inactivation of MAPK activity involved in osmolarity sensing and associated with cell wall integrity; also implicated in pheromone-dependent signal transduction, mitochondrion inheritance, and tRNA splicing via endonucleolytic cleavage and ligation; localizes to the nucleus and cytoplasm
View computational annotations
Molecular Function
- Manually Curated
- enables MAP kinase serine/threonine phosphatase activity (IDA)
- enables protein binding (IPI)
- enables protein serine/threonine phosphatase activity (IDA)
Biological Process
- Manually Curated
- involved in fungal-type cell wall organization or biogenesis (IGI, IMP)
- involved in mitochondrion inheritance (IMP)
- involved in negative regulation of MAPK cascade (IGI, IMP)
- involved in pheromone-dependent signal transduction involved in conjugation with cellular fusion (IMP)
- involved in tRNA splicing, via endonucleolytic cleavage and ligation (IMP)
Phenotype
Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided. Click "Phenotype Details" to view all phenotype annotations and evidence for this locus as well as phenotypes it shares with other genes.
- Summary
- PTC1/YDL006W is a non-essential gene; null mutants are viable but exhibit a range of phenotypic changes including decreased alkaline pH resistance, increased autophagy, auxotrophy, abnormal budding pattern, arrested cell cycle progression in the G1 phase, decreased competitive fitness, decreased cytokinesis, abnormal endoplasmic reticulum morphology, increased heat sensitivity, decreased ionic stress resistance, increased killer toxin resistance, abnormal lipid particle morphology, decreased metal resistance, abnormal mitochondrial distribution and morphology, decreased oxidative stress resistance, abnormal protein/peptide distribution and modification, decreased replicative lifespan, decreased resistance to enzymatic treatment, decreased respiratory growth rate, decreased small molecule transport, decreased sporulation, decreased telomere length, decreased toxin resistance, decreased utilization of carbon sources, abnormal vacuolar morphology, and decreased vegetative growth rate.
Classical Genetics
- unspecified
- null
- autophagy: increased
- budding pattern: abnormal
- cell cycle progression in G1 phase: arrested
- chemical compound accumulation: decreased
- chemical compound accumulation: increased
- cytokinesis: decreased
- endoplasmic reticulum morphology: abnormal
- heat sensitivity: increased
- ionic stress resistance: decreased
- killer toxin resistance: increased
- metal resistance: decreased
- mitochondrial distribution: abnormal
- mitochondrial morphology: abnormal
- protein/peptide accumulation: decreased
- protein/peptide accumulation: increased
- protein/peptide distribution: abnormal
- protein/peptide modification: decreased
- protein/peptide modification: increased
- resistance to chemicals: decreased
- resistance to enzymatic treatment: decreased
- small molecule transport: decreased
- vacuolar morphology: abnormal
- null
- alkaline pH resistance: decreased
- auxotrophy
- chemical compound accumulation: abnormal
- chemical compound accumulation: decreased
- chemical compound accumulation: increased
- competitive fitness: decreased
- heat sensitivity: increased
- lipid particle morphology: abnormal
- metal resistance: decreased
- mitochondrial morphology: abnormal
- oxidative stress resistance: decreased
- replicative lifespan: decreased
- resistance to chemicals: decreased
- resistance to chemicals: increased
- resistance to enzymatic treatment: decreased
- respiratory growth: decreased rate
- sporulation: decreased
- telomere length: decreased
- toxin resistance: decreased
- utilization of carbon source: decreased
- vegetative growth: abnormal
- vegetative growth: decreased rate
- viable
Large-scale Survey
Interaction
Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. An interaction annotation is composed of the interaction type, name of the interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a reference, as well as other experimental details. Click "Interaction Details" to view all interaction annotations and evidence for this locus, including an interaction visualization.
- Summary
- Ptc1p interacts physically with proteins involved in cell cycle regulation; PTC1 interacts genetically with genes involved in transcription
1420 total interactions for 839 unique genes
Physical Interactions
- Affinity Capture-MS: 14
- Affinity Capture-RNA: 4
- Affinity Capture-Western: 5
- Biochemical Activity: 13
- Co-localization: 1
- Co-purification: 1
- PCA: 19
- Proximity Label-MS: 1
- Reconstituted Complex: 6
- Two-hybrid: 6
Genetic Interactions
- Dosage Growth Defect: 1
- Dosage Lethality: 1
- Dosage Rescue: 37
- Negative Genetic: 1013
- Phenotypic Enhancement: 4
- Phenotypic Suppression: 21
- Positive Genetic: 186
- Synthetic Growth Defect: 50
- Synthetic Haploinsufficiency: 1
- Synthetic Lethality: 21
- Synthetic Rescue: 15
Regulation
The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the given locus, based on experimental evidence. This evidence includes data generated through high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO enrichment among regulation Targets, and a regulator/target diagram for the locus.
Expression
Expression data are derived from records contained in the Gene Expression Omnibus (GEO), and are first log2 transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result there may be a greater number of conditions than datasets represented in a single clickable histogram bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from those that are up-regulated (red). Click "Expression Details" to view all expression annotations and details for this locus, including a visualization of genes that share a similar expression pattern.
Summary Paragraph
A summary of the locus, written by SGD Biocurators following a thorough review of the literature. Links to gene names and curated GO terms are included within the Summary Paragraphs.
Last Updated: 2024-12-05
Literature
All manually curated literature for the specified gene, organized into topics according to their relevance to the gene (Primary Literature, Additional Literature, or Review). Click "Literature Details" to view all literature information for this locus, including shared literature between genes.