RAD27 / YKL113C Overview
- Standard Name
- RAD27 1
- Systematic Name
- YKL113C
- SGD ID
- SGD:S000001596
- Aliases
- ERC11 27 , RTH1 10 , FEN1 28
- Feature Type
- ORF , Verified
- Description
- 5' to 3' exonuclease, 5' flap endonuclease; required for Okazaki fragment processing and maturation, for long-patch base-excision repair and large loop repair (LLR), ribonucleotide excision repair; cleaves off RNA flap in a RNA:DNA hybrid intermediate during processing of DNA lagging strand; relocalizes to the cytosol in response to hypoxia; member of the S. pombe RAD2/FEN1 family 2 3 4 5 6 7
- Name Description
- RADiation sensitive 1
- Comparative Info
-
Sequence
The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains.
Analyze Sequence
S288C only
BLASTN | BLASTP | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species
BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi
S288C vs. other strains
Protein
Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.
- Summary
- Rad27p is 382 amino acids long, shorter-lived, low in abudance; contains 5 XPG/Rad2 endonuclease sites; ubiquitinylated on K81, acetylated on K246, phosphorylated on T193 and S302; relocalizes to the cytosol in response to hypoxia
- Length (a.a.)
- 382
- Mol. Weight (Da)
- 43289.8
- Isoelectric Point
- 9.37
- Median Abundance (molecules/cell)
- 3492 +/- 776
- Half-life (hr)
- 8.8
Alleles
Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results.
View all RAD27 alleles in SGD search
Gene Ontology
GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes.
- Summary
- 5-3 exonuclease and 5-flap endonuclease involved in base-free sugar phosphate removal as a part of base-excision repair, DNA repair synthesis during gene conversion at the mating-type locus, removal of RNA primers during DNA replication, maintenance of DNA trinucleotide repeats, and non-homologous end joining; also involved in replicative cell aging; localizes to the nucleus, mitochondrion, and cytosol
View computational annotations
Molecular Function
- Manually Curated
- enables 5'-3' exonuclease activity (IDA)
- enables 5'-flap endonuclease activity (IDA)
Biological Process
- Manually Curated
- involved in DNA replication, removal of RNA primer (IDA)
- involved in double-strand break repair via nonhomologous end joining (IDA)
- involved in gene conversion at mating-type locus (IMP)
- involved in maintenance of DNA trinucleotide repeats (IGI, IMP)
Phenotype
Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided. Click "Phenotype Details" to view all phenotype annotations and evidence for this locus as well as phenotypes it shares with other genes.
- Summary
- Non-essential gene; null mutant displays sensitivity to agents affecting DNA metabolism such as 5-fluorouracil, MMS, and UV light; null mutant has elevated mutation frequency, decreased replicative lifespan, and abnormal nuclear morphology; in large-scale studies null mutant displays decreased competitive fitness, chronological lifespan, and filamentous growth, and is sensitive to heat, oxidative stress, metals, and various chemicals
Classical Genetics
- null
- cell size: increased
- chromosome/plasmid maintenance: abnormal
- chromosome/plasmid maintenance: decreased
- colony sectoring: increased
- growth in exponential phase: decreased rate
- heat sensitivity: increased
- metal resistance: decreased
- mitotic recombination: increased
- mitotic recombination: increased rate
- mutation frequency: increased
- nuclear morphology: abnormal
- protein/peptide distribution: abnormal
- replicative lifespan: decreased
- resistance to chemicals: decreased
- resistance to chemicals: increased
- transposable element transposition: increased
- UV resistance: decreased
- reduction of function
- null
- bud morphology: abnormal
- budding index: increased
- chronological lifespan: decreased
- competitive fitness: decreased
- fermentative growth: decreased rate
- filamentous growth: decreased
- heat sensitivity: increased
- invasive growth: absent
- killer toxin resistance: decreased
- metal resistance: decreased
- mitotic recombination: increased
- mutation frequency: increased
- nuclear morphology: abnormal
- oxidative stress resistance: decreased
- protein/peptide accumulation: increased
- replicative lifespan: decreased
- resistance to chemicals: decreased
- resistance to chemicals: increased
- resistance to enzymatic treatment: decreased
- resistance to enzymatic treatment: increased
- silencing: decreased
- starvation resistance: decreased
- telomere length: increased
- toxin resistance: decreased
- transposable element transposition: increased
- vegetative growth: decreased rate
- viable
- overexpression
Large-scale Survey
Disease
Disease Annotations consist of three mandatory components: a gene product, a term from the Disease Ontology (DO) controlled vocabulary and an evidence code. SGD provides manually curated DO Annotations derived from the literature. Click "Disease Details" to view all Disease information and evidence for this locus as well as diseases it shares with other genes.
Interaction
Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. An interaction annotation is composed of the interaction type, name of the interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a reference, as well as other experimental details. Click "Interaction Details" to view all interaction annotations and evidence for this locus, including an interaction visualization.
- Summary
- Rad27p interacts physically with proteins involved in DNA repair; RAD27 interacts genetically with genes involved in DNA repair
1445 total interactions for 631 unique genes
Physical Interactions
- Affinity Capture-MS: 12
- Affinity Capture-RNA: 2
- Affinity Capture-Western: 8
- Reconstituted Complex: 10
- Two-hybrid: 3
Genetic Interactions
- Dosage Growth Defect: 4
- Dosage Lethality: 4
- Dosage Rescue: 13
- Negative Genetic: 708
- Phenotypic Enhancement: 57
- Phenotypic Suppression: 13
- Positive Genetic: 71
- Synthetic Growth Defect: 244
- Synthetic Lethality: 283
- Synthetic Rescue: 13
Regulation
The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the given locus, based on experimental evidence. This evidence includes data generated through high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO enrichment among regulation Targets, and a regulator/target diagram for the locus.
- Regulators
- 3
- Targets
- 0
Expression
Expression data are derived from records contained in the Gene Expression Omnibus (GEO), and are first log2 transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result there may be a greater number of conditions than datasets represented in a single clickable histogram bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from those that are up-regulated (red). Click "Expression Details" to view all expression annotations and details for this locus, including a visualization of genes that share a similar expression pattern.
Summary Paragraph
A summary of the locus, written by SGD Biocurators following a thorough review of the literature. Links to gene names and curated GO terms are included within the Summary Paragraphs.
Last Updated: 2007-10-05
Literature
All manually curated literature for the specified gene, organized into topics according to their relevance to the gene (Primary Literature, Additional Literature, or Review). Click "Literature Details" to view all literature information for this locus, including shared literature between genes.