CSG2 / YBR036C Overview
- Standard Name
- CSG2 1
- Systematic Name
- YBR036C
- SGD ID
- SGD:S000000240
- Aliases
- CLS2 13
- Feature Type
- ORF , Verified
- Description
- Calcium release channel involved in ER calcium homeostasis; integrates ER calcium release with the regulation of sphingolipid metabolism and autophagy; required for mannosylation of inositolphosphorylceramide and for growth at high calcium concentrations; endoplasmic reticulum membrane protein; contains 10 transmembrane domains and a potential Ca2+ EF-hand motif; protein abundance increases in response to DNA replication stress 1 2 3 4 5
- Name Description
- Calcium Sensitive Growth 1
- Comparative Info
-
Sequence
The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains.
Analyze Sequence
S288C only
BLASTN | BLASTP | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species
BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi
S288C vs. other strains
Protein
Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.
- Summary
- Protein abundance increases in response to DNA replication stress
- Length (a.a.)
- 410
- Mol. Weight (Da)
- 45442.5
- Isoelectric Point
- 7.33
- Median Abundance (molecules/cell)
- 3749 +/- 1820
Alleles
Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results.
View all CSG2 alleles in SGD search
Gene Ontology
GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes.
- Summary
- Regulatory subunit for mannosylinositol phosphorylceramide (MIPC) synthase; required for biosynthesis of mature sphingolipids; integral to the ER membranes
View computational annotations
Molecular Function
- Manually Curated
- enables enzyme regulator activity (IMP)
- enables intracellularly gated calcium channel activity (IDA)
Biological Process
- Manually Curated
- involved in endoplasmic reticulum calcium ion homeostasis (IMP)
- involved in glycosphingolipid biosynthetic process (IMP)
- involved in intracellular calcium ion homeostasis (IMP)
- involved in release of sequestered calcium ion into cytosol by endoplasmic reticulum (IMP)
Cellular Component
- Manually Curated
- located in endoplasmic reticulum (HDA)
- is active in endoplasmic reticulum membrane (IDA)
- located in fungal-type vacuole (HDA)
Complex
Macromolecular complex annotations are imported from the Complex Portal. These annotations have been derived from physical molecular interaction evidence extracted from the literature and cross-referenced in the entry, or by curator inference from information on homologs in closely related species or by inference from scientific background.
Pathways
Phenotype
Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided. Click "Phenotype Details" to view all phenotype annotations and evidence for this locus as well as phenotypes it shares with other genes.
- Summary
- Non-essential gene; null mutant accumulates unmannosylated sphingolipids (ceramide and inositol phosphoceramide, IPC) instead of mannosylated forms, MIPC and M(IP)2C; mutants accumulate calcium and show increased sensitivity to extracellular calcium; in systematic studies mutants show decreased competitive fitness and sensitivity to multiple chemicals, including ethanol, acetaldehyde, hydrogen peroxide, hydroxyurea, caffeine, tunicamycin, azoles
Classical Genetics
- null
- acid pH resistance: decreased
- autophagy: absent
- chemical compound accumulation: decreased
- chemical compound accumulation: increased
- metal resistance: decreased
- protein transport: abnormal
- protein/peptide accumulation: decreased
- protein/peptide distribution: abnormal
- protein/peptide modification: increased
- resistance to chemicals: decreased
- resistance to chemicals: increased
- small molecule transport: decreased
- starvation resistance: decreased
- viable
- unspecified
- null
- acid pH resistance: decreased
- biofilm formation: decreased
- chemical compound accumulation: decreased
- chemical compound excretion: increased
- competitive fitness: decreased
- heat sensitivity: increased
- hyperosmotic stress resistance: decreased
- killer toxin resistance: decreased
- metal resistance: decreased
- oxidative stress resistance: increased
- resistance to chemicals: decreased
- resistance to chemicals: increased
- respiratory growth: absent
- stress resistance: decreased
- toxin resistance: decreased
- utilization of nitrogen source: decreased rate
- vacuolar morphology: abnormal
- viable
Large-scale Survey
Disease
Disease Annotations consist of three mandatory components: a gene product, a term from the Disease Ontology (DO) controlled vocabulary and an evidence code. SGD provides manually curated DO Annotations derived from the literature. Click "Disease Details" to view all Disease information and evidence for this locus as well as diseases it shares with other genes.
- Summary
- Yeast CSG2 has been used to study the role of human GDAP1 in structure and function of the trans-Golgi network and how that relates to Charcot-Marie-Tooth disease
Manually Curated
Interaction
Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. An interaction annotation is composed of the interaction type, name of the interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a reference, as well as other experimental details. Click "Interaction Details" to view all interaction annotations and evidence for this locus, including an interaction visualization.
1286 total interactions for 981 unique genes
Physical Interactions
- Affinity Capture-MS: 4
- Affinity Capture-RNA: 5
- Affinity Capture-Western: 2
- PCA: 44
Genetic Interactions
- Dosage Growth Defect: 1
- Dosage Lethality: 2
- Dosage Rescue: 3
- Negative Genetic: 947
- Phenotypic Enhancement: 4
- Phenotypic Suppression: 3
- Positive Genetic: 204
- Synthetic Growth Defect: 26
- Synthetic Lethality: 10
- Synthetic Rescue: 31
Regulation
The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the given locus, based on experimental evidence. This evidence includes data generated through high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO enrichment among regulation Targets, and a regulator/target diagram for the locus.
- Regulators
- 5
- Targets
- 0
Expression
Expression data are derived from records contained in the Gene Expression Omnibus (GEO), and are first log2 transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result there may be a greater number of conditions than datasets represented in a single clickable histogram bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from those that are up-regulated (red). Click "Expression Details" to view all expression annotations and details for this locus, including a visualization of genes that share a similar expression pattern.
Summary Paragraph
A summary of the locus, written by SGD Biocurators following a thorough review of the literature. Links to gene names and curated GO terms are included within the Summary Paragraphs.
Last Updated: 2007-10-05
Literature
All manually curated literature for the specified gene, organized into topics according to their relevance to the gene (Primary Literature, Additional Literature, or Review). Click "Literature Details" to view all literature information for this locus, including shared literature between genes.