TEF2 / YBR118W Overview
- Standard Name
- TEF2 1
- Systematic Name
- YBR118W
- SGD ID
- SGD:S000000322
- Aliases
- eEF1A 7 , EF-1 alpha
- Feature Type
- ORF , Verified
- Description
- Translational elongation factor EF-1 alpha; GTP-bound active form, binds to and delivers aminoacylated tRNA to the A-site of ribosomes for elongation of nascent polypeptides; moonlighting function as an actin binding and bundling protein; association with GTPase Rho1p on the vacuolar membrane may facilitate F-actin remodeling; involved in tRNA re-export from the nucleus; Tef2p-RFP levels increase during replicative aging 1 2 3 4 5 6
- Name Description
- Translation Elongation Factor 1
- Paralog
- TEF1
- Comparative Info
-
Sequence
The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains.
- Summary
- TEF2/YBR118W is located on the right arm of chromosome II between TKL2 transketolase and MUD1 U1 snRNP A protein; coding sequence is 1377 nucleotides long with 3 synonymous SNPs; TEF2 has a paralog, TEF1, that arose from the whole genome duplication
Analyze Sequence
S288C only
BLASTN | BLASTP | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species
BLASTN vs. fungi | BLASTP at NCBI | BLASTP at NCBI | BLASTP vs. fungi
S288C vs. other strains
Protein
Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.
- Summary
- Tef1p is 458 amino acids long, and extremely abundant (eEF1A is the most abundant protein in the eukaryotic cell); contains 5 translational (tr)-type GTP-binding domains; methylated on 7 lysines, acetylated on 19 lysines, succinylated on 22 lysines, sumoylated on 12 lysines, ubiquitinylated on 27 lysines, phosphorylated on 28 residues
- Length (a.a.)
- 458
- Mol. Weight (Da)
- 50045.3
- Isoelectric Point
- 9.58
- Median Abundance (molecules/cell)
- 532551 +/- 389013
Alleles
Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results.
View all TEF2 alleles in SGD search
Gene Ontology
GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes.
- Summary
- GTP- and GDP-binding translation elongation factor; subunit of translation elongation factor 1 complex that binds GTP and aminoacyl-tRNAs, and catalyzes their codon-dependent placement at the A-site of the ribosome; involved in the transport of tRNAs from nucleus to cytoplasm; moonlighting role as an actin filament bundling; associates with the Rho1p GTPase in the vacuole membrane; also localizes to mitochondria and vacuolar membranes
View computational annotations
Molecular Function
- Manually Curated
- enables translation elongation factor activity (IMP)
Biological Process
- Manually Curated
- involved in translational elongation (IMP)
Phenotype
Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided. Click "Phenotype Details" to view all phenotype annotations and evidence for this locus as well as phenotypes it shares with other genes.
- Summary
- Non-essential gene; null mutants are sensitive to TOR inhibitor rapamycin; heterozygous diploid null mutants are heat-tolerant, have unstable chromosomes, and are sensitive to various antibiotics; overexpression increases cell size, interferes with the cell cycle, slows growth, and disturbs the actin cytoskeleton
Classical Genetics
Disease
Disease Annotations consist of three mandatory components: a gene product, a term from the Disease Ontology (DO) controlled vocabulary and an evidence code. SGD provides manually curated DO Annotations derived from the literature. Click "Disease Details" to view all Disease information and evidence for this locus as well as diseases it shares with other genes.
- Summary
- Yeast TEF2 is homologous to human EEF1A2, and has been used to study mutations found in patients with epileptic-dyskinetic encephalopathy with multisystem involvement including cardiomyopathy, epilepsy, global developmental delay, and early death
Manually Curated
Interaction
Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. An interaction annotation is composed of the interaction type, name of the interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a reference, as well as other experimental details. Click "Interaction Details" to view all interaction annotations and evidence for this locus, including an interaction visualization.
- Summary
- Tef2p interacts physically with proteins involved in translation and organelle organization; TEF2 interacts genetically with genes involved in transcription; the tef2 null mutant is viable, the null mutant of paralog tef1 is viable, the tef2 tef1 double mutant is inviable
479 total interactions for 377 unique genes
Physical Interactions
- Affinity Capture-MS: 115
- Affinity Capture-RNA: 7
- Affinity Capture-Western: 12
- Biochemical Activity: 12
- Co-crystal Structure: 2
- Co-fractionation: 4
- Co-purification: 1
- Cross-Linking-MS (XL-MS): 9
- PCA: 2
- Reconstituted Complex: 7
- Two-hybrid: 2
Genetic Interactions
- Dosage Growth Defect: 1
- Dosage Lethality: 2
- Dosage Rescue: 7
- Negative Genetic: 227
- Phenotypic Enhancement: 1
- Phenotypic Suppression: 5
- Positive Genetic: 50
- Synthetic Growth Defect: 5
- Synthetic Lethality: 6
- Synthetic Rescue: 2
Regulation
The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the given locus, based on experimental evidence. This evidence includes data generated through high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO enrichment among regulation Targets, and a regulator/target diagram for the locus.
- Summary
- TEF2 promoter is bound by Fhl1p, Med2p, Rgr1p, Spt6p, and Xbp1p in response to heat; TEF2 transcription is upregulated by Ifh1p, and is upregulated by Znf1p during xylose fermentation
- Regulators
- 17
- Targets
- 0
Expression
Expression data are derived from records contained in the Gene Expression Omnibus (GEO), and are first log2 transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result there may be a greater number of conditions than datasets represented in a single clickable histogram bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from those that are up-regulated (red). Click "Expression Details" to view all expression annotations and details for this locus, including a visualization of genes that share a similar expression pattern.
Summary Paragraph
A summary of the locus, written by SGD Biocurators following a thorough review of the literature. Links to gene names and curated GO terms are included within the Summary Paragraphs.
Last Updated: 2024-07-09
Literature
All manually curated literature for the specified gene, organized into topics according to their relevance to the gene (Primary Literature, Additional Literature, or Review). Click "Literature Details" to view all literature information for this locus, including shared literature between genes.