HSL7 / YBR133C Overview
- Standard Name
- HSL7 1
- Systematic Name
- YBR133C
- SGD ID
- SGD:S000000337
- Feature Type
- ORF , Verified
- Description
- Protein arginine N-methyltransferase; exhibits septin and Hsl1p-dependent localization to the bud neck in budded cells and periodic Hsl1p-dependent phosphorylation; required with Hsl1p, and Elm1p for the mother-bud neck recruitment, phosphorylation, and degradation of Swe1p; responsible for symmetric dimethylation of histone H4 on Arg3; relocalizes away from bud neck upon DNA replication stress; human homolog PRMT5 can complement yeast hsl7 mutant 2 3 4 5 6 7 8 9 10
- Name Description
- Histone Synthetic Lethal 1
- Comparative Info
-
Sequence
The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains.
Analyze Sequence
S288C only
BLASTN | BLASTP | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species
BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi
S288C vs. other strains
Protein
Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.
- Length (a.a.)
- 827
- Mol. Weight (Da)
- 95139.2
- Isoelectric Point
- 6.31
- Median Abundance (molecules/cell)
- 1877 +/- 697
- Half-life (hr)
- 4.9
Alleles
Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results.
View all HSL7 alleles in SGD search
Gene Ontology
GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes.
- Summary
- Protein-arginine N-methyltransferase involved in protein catabolism and regulation of the G2/M transition of mitosis; localizes to the septin collar of the bud neck, and the outer plaque of the spindle pole body in the G1 phase of the cell cycle
View computational annotations
Molecular Function
- Manually Curated
Biological Process
- Manually Curated
- involved in G2/M transition of mitotic cell cycle (IMP)
- involved in protein catabolic process (IMP)
- involved in regulation of cell cycle (IMP)
Cellular Component
- Manually Curated
- located in cellular bud neck (HDA, IDA)
- located in cellular bud neck septin collar (IDA)
- located in cellular bud neck septin ring (IDA, IMP)
- located in cytoplasm (HDA)
- located in outer plaque of spindle pole body (IDA)
Phenotype
Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided. Click "Phenotype Details" to view all phenotype annotations and evidence for this locus as well as phenotypes it shares with other genes.
- Summary
- Non-essential gene; null mutant has decreased growth rate in rich media and displays strain specific (W303) elongated buds with an undivided nucleus in the bud neck; haploid null displays increased invasive growth, and homozygous diploid null has elevated pseudohyphal growth; null mutant has decreased chronological lifespan, desiccation resistance, and thermotolerance; Swe1p is stabilized and less hyperphosphorylated during G2/M phase in the haploid null mutant; haploinsufficiency in the heterozygous diploid null results in elevated chromosomal instability; in large-scale studies the null mutant displays reduced competitive fitness, decreased sporulation and elevated oxidative stress resistance
Classical Genetics
- null
- overexpression
- null
- auxotrophy
- chemical compound accumulation: abnormal
- chemical compound accumulation: decreased
- chemical compound accumulation: increased
- chromosome/plasmid maintenance: decreased
- chronological lifespan: decreased
- competitive fitness: decreased
- desiccation resistance: decreased
- haploinsufficient
- innate thermotolerance: decreased
- killer toxin resistance: increased
- metal resistance: increased
- oxidative stress resistance: increased
- resistance to chemicals: decreased
- resistance to chemicals: increased
- resistance to chemicals: normal
- sporulation: decreased
- vacuolar morphology: abnormal
- vegetative growth: abnormal
- vegetative growth: decreased rate
- viable
Large-scale Survey
Interaction
Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. An interaction annotation is composed of the interaction type, name of the interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a reference, as well as other experimental details. Click "Interaction Details" to view all interaction annotations and evidence for this locus, including an interaction visualization.
162 total interactions for 128 unique genes
Physical Interactions
- Affinity Capture-MS: 11
- Affinity Capture-RNA: 5
- Affinity Capture-Western: 13
- Biochemical Activity: 5
- Co-localization: 1
- Co-purification: 1
- Reconstituted Complex: 4
- Two-hybrid: 10
Genetic Interactions
- Dosage Rescue: 2
- Negative Genetic: 83
- Phenotypic Suppression: 4
- Positive Genetic: 7
- Synthetic Growth Defect: 5
- Synthetic Haploinsufficiency: 1
- Synthetic Lethality: 6
- Synthetic Rescue: 4
Regulation
The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the given locus, based on experimental evidence. This evidence includes data generated through high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO enrichment among regulation Targets, and a regulator/target diagram for the locus.
- Regulators
- 11
- Targets
- 0
Expression
Expression data are derived from records contained in the Gene Expression Omnibus (GEO), and are first log2 transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result there may be a greater number of conditions than datasets represented in a single clickable histogram bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from those that are up-regulated (red). Click "Expression Details" to view all expression annotations and details for this locus, including a visualization of genes that share a similar expression pattern.
Literature
All manually curated literature for the specified gene, organized into topics according to their relevance to the gene (Primary Literature, Additional Literature, or Review). Click "Literature Details" to view all literature information for this locus, including shared literature between genes.