PAF1 / YBR279W Phenotype

Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided.

Summary

PAF1/YBR279W is a non-essential gene, null mutants are viable and associated with a variety of phenotypes that significantly impact cellular functions and overall fitness. Mutants lacking PAF1 exhibit a decreased rate of anaerobic growth, indicating a compromised ability to thrive in low-oxygen environments. These mutants display abnormal bud morphology and experience delays in cell cycle progression during the G1 phase, which can hinder normal cell division. Cell size is increased, yet the chronological lifespan is decreased, suggesting a trade-off between growth and longevity. Cold sensitivity is heightened, making these mutants more vulnerable to lower temperatures, while competitive fitness is diminished, leading to reduced viability in resource-limited conditions. Resistance to desiccation, hydrostatic pressure, and killer toxins is decreased, further underscoring their compromised resilience. The growth rate during the exponential phase is also reduced, and heat sensitivity is increased, indicating greater susceptibility to elevated temperatures. Although oxidative stress resistance is increased, the replicative lifespan is extended, which may reflect a complex relationship between stress responses and cellular aging. However, the mutants show an absent mating response and sporulation, indicating disruptions in reproductive capabilities. Metal resistance is decreased, and the morphology of lipid particles is abnormal, which could affect cellular metabolism. Telomere length is reduced, and transposable element transposition is increased, suggesting potential genomic instability. Vacuolar morphology is also abnormal, impacting cellular storage and waste management. Overexpression of PAF1 decreases growth rate during the exponential phase, highlighting the gene's intricate role in regulating growth and cellular processes.

Annotations

A phenotype is defined as an observable (e.g., apoptosis) and a qualifier (e.g., increased). There may be more than one row with the same phenotype if that phenotype was observed in separate studies or in different conditions, strains, alleles, etc.

Shared Phenotypes

This diagram displays phenotype observables (purple squares) that are shared between the given gene (yellow circle) and other genes (gray circles) based on the number of phenotype observables shared (adjustable using the slider at the bottom).

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