CHA1 / YCL064C Overview
- Standard Name
- CHA1 1
- Systematic Name
- YCL064C
- SGD ID
- SGD:S000000569
- Feature Type
- ORF , Verified
- Description
- Catabolic L-serine (L-threonine) deaminase; catalyzes the degradation of both L-serine and L-threonine; required to use serine or threonine as the sole nitrogen source, transcriptionally induced by serine and threonine 1 2
- Name Description
- Catabolism of Hydroxy Amino acids 1
- Comparative Info
-
Sequence
The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains.
- Summary
- CHA1/YCL064C is located on the left arm of chromosome III near the telomere between HML mating type cassette and VAC17; coding sequence is 1083 amino acids long with 11 SNPs, 3 of which cause amino acid polymorphisms
Analyze Sequence
S288C only
BLASTN | BLASTP | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species
BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi
S288C vs. other strains
Protein
Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.
- Summary
- Cha1p is 360 amino acids long, extremely short-lived, low in abundance; contains Arg/Lys polymorphism at residue 58, Ser/Phe at residue 59, and Asp/His at residue 264
- Length (a.a.)
- 360
- Mol. Weight (Da)
- 39309.9
- Isoelectric Point
- 9.01
- Median Abundance (molecules/cell)
- 8233 +/- 4142
- Half-life (hr)
- 1.5
Alleles
Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results.
View all CHA1 alleles in SGD search
Gene Ontology
GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes.
- Summary
- Serine and threonine deaminase; catalyzes the catabolism of hydroxy amino acids; localizes to the mitochondrial nucleoid
View computational annotations
Molecular Function
- Manually Curated
- enables L-serine ammonia-lyase activity (IDA)
- enables threonine deaminase activity (IDA)
Biological Process
- Manually Curated
- involved in L-serine catabolic process (IGI)
- involved in threonine catabolic process (IGI)
Cellular Component
- Manually Curated
- located in mitochondrial nucleoid (IDA)
- located in mitochondrion (HDA)
Pathways
Phenotype
Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided. Click "Phenotype Details" to view all phenotype annotations and evidence for this locus as well as phenotypes it shares with other genes.
- Summary
- CHA1/YCL064C is a non-essential gene; null mutants are viable and show increased colony sectoring, increased heat tolerance, decreaed competitive fitness, and defective in utilization of serine or threonine as nitrogen sources; overexpression reduces UV resistance
Classical Genetics
Interaction
Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. An interaction annotation is composed of the interaction type, name of the interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a reference, as well as other experimental details. Click "Interaction Details" to view all interaction annotations and evidence for this locus, including an interaction visualization.
- Summary
- Cha1p interacts physically with proteins involved in cellular respiration and amino acid metabolism; CHA1 interacts genetically with genes involved in transcription
130 total interactions for 115 unique genes
Physical Interactions
- Affinity Capture-MS: 72
- Affinity Capture-RNA: 3
- Co-fractionation: 1
- Proximity Label-MS: 3
Genetic Interactions
- Dosage Rescue: 1
- Negative Genetic: 35
- Phenotypic Suppression: 1
- Positive Genetic: 7
- Synthetic Rescue: 7
Regulation
The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the given locus, based on experimental evidence. This evidence includes data generated through high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO enrichment among regulation Targets, and a regulator/target diagram for the locus.
- Summary
- CHA1/YCL064C promoter is bound by 10 different transcription factors in response to heat, and by Fkh1p in response to starvation; CHA1 transcription is downregulated by Sir2p, Sir3p, and Sir4p during mitotic cell cycle, and upregulated by Znf1p during xylose fermentation
- Regulators
- 35
- Targets
- 0
Expression
Expression data are derived from records contained in the Gene Expression Omnibus (GEO), and are first log2 transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result there may be a greater number of conditions than datasets represented in a single clickable histogram bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from those that are up-regulated (red). Click "Expression Details" to view all expression annotations and details for this locus, including a visualization of genes that share a similar expression pattern.
Summary Paragraph
A summary of the locus, written by SGD Biocurators following a thorough review of the literature. Links to gene names and curated GO terms are included within the Summary Paragraphs.
Last Updated: 2025-03-05
Literature
All manually curated literature for the specified gene, organized into topics according to their relevance to the gene (Primary Literature, Additional Literature, or Review). Click "Literature Details" to view all literature information for this locus, including shared literature between genes.